Comparative antimicrobial activity of Cymbopogon citratus essential oil and thiosemicarbazones derived from this oil
1 Laboratory of Pharmacognosy and Essential Oils, Faculty of Sciences and Techniques/University of Abomey-Calavi (UAC) Cotonou, Republic of Benin.
2 Kaba Research Laboratory in Chemistry and Applications (LaKReCA), University of Technical Sciences, Engineering and Mathematics (UNSTIM), Abomey, Republic of Benin.
3 Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, University of Abomey-Calavi, Cotonou, Republic of Benin.
4 College of Agroforestry, Agrovalorisation Laboratory, Department of Biochemistry and Microbiology, Jean Lorougnon GUEDE University, Côte d'Ivoire.
Research Article
GSC Advanced Research and Reviews, 2021, 09(03), 084–092.
Article DOI: 10.30574/gscarr.2021.9.3.0300
Publication history:
Received on 19 November 2021; revised on 21 December 2021; accepted on 23 December 2021
Abstract:
Introduction: The presence of microbes in our environment is always a permanent public health problem. In this context, research on natural treatment, less expensive and accessible to fight these microbial germs would be beneficial.
Methods: During this work, molecules of thiosemicarbazones due to their numerous biological activities were hemi-synthesized in situ in the essential oil of Cymbopogon citratus in order to evaluate their antimicrobial activities.
Results: Analysis of the essential oil extracted by hydrodistillation revealed the presence of 72.91% of citral. Citralthiosemicarbazone (CThio) and citral 4-phenyl-3-thiosemicarbazone (CPthio) were hemi-synthesized in this oil with interesting yields of 83% and 91%, respectively. After purification and confirmation of the structures of these molecules, the three substances were tested on eleven strains of microbes. Determination of the inhibition diameters showed that the activity of the essential oil is best in over 80% of strains. However, the largest diameter of inhibition (26 mm) was noted with CPthio against Salmonella typhi R 30951401. The determination of the minimum inhibitory concentrations showed that the oil remains more active with the smallest value of 0.3125 mg / ml against Micrococcus luteus. The lower value of minimum bactericidal concentrations was also obtained with the essential oil against Enterococcus foecalis ATCC 29212.
Conclusion: The essential oil of C. citratus remains more active in the majority cases. It could be a great alternative in the fight against bacteria, and the advantage is that it remains a natural substance.
Keywords:
Thiosemicarbazones; Hemi-synthesized; Cymbopogon citratus; Antimicrobial activities; Minimum inhibitory concentrations; Minimum bactericidal concentrations.
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Copyright © 2021 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
