Association of Methylenetetrahydrofolate Reductase (MTHFR) Gene C677T (rs1801133) polymorphism among Sudanese patients with chronic myelogenous leukemia
1 College of Medical Laboratory Sciences, Department of Hematology, Karary University, Khartoum Sudan.
2 College of Health Sciences, Department of Medical Laboratory Sciences, Gulf Medical University, AL JURF, Ajman, United Arab Emirates.
Research Article
GSC Biological and Pharmaceutical Sciences, 2023, 22(03), 218–224.
Article DOI: 10.30574/gscbps.2023.22.3.0091
Publication history:
Received on 01 February 2023; revised on 10 March 2023; accepted on 13 March 2023
Abstract:
Background: Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme regulating the intracellular folate metabolism which plays an important role in carcinogenesis through DNA methylation.
Objectives: The current study aimed to determine the association between MTHFR C677T polymorphisms and the risks of chronic myeloid leukemia (CML).
Material and methods: This are hospital based cross sectional case control study, a total of 170 Sudanese subjects were enrolled, and 75 patients with chronic myeloid leukemia and 75 age- and sex matched healthy volunteers as a control group. Genomic DNA was extracted by sodium chloride method and The SNPs genotypes were determined using polymerase chain reaction followed by restriction fragment length polymorphism method. (PCR- RFLP). Data of this study were collected using a structured interview questionnaire and analyzed by statistical package for social sciences (version 21).
Results: The frequency of the CC genotype was higher in the control group compared with patients (100%,96%), while of the CT genotype was higher in the case (4%) than in control (0.0%); the TT genotype was absent in both study groups, the frequencies T and C allele were 0.02 and 0.98 in CML patient group and 0.00, 0.100 respectively in the control group. There was no clinically significant association between age (P.value= 0.3), gender (P.value= 0.4) and genotype, also there was no statistically significant association when compare case with control genotype (p. value 0.08).
Conclusion: C677T MTHFR polymorphism is not associated with the risk of CML among the Sudanese population
Keywords:
Leukemia; Methylenetetrahydrofolate reductase; Polymorphism; Sudan
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