The capability of Piper betle leaves ethanolic extract to inhibit neutrophil infiltration in sepsis-induced BALB/c Mice
1 Department of Medical Science, Faculty of Medicine, Universitas Negeri Surabaya, Surabaya, East Java, 60213, Indonesia.
2 Department of Pediatrics of dr. Saiful Anwar General Hospital, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, 65145, Indonesia.
3 Medical Degree, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, 65145, Indonesia.
4 Department of Clinical Microbiology of dr. Saiful Anwar General Hospital, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, 65145, Indonesia.
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 26(03), 212–216.
Article DOI: 10.30574/gscbps.2024.26.3.0103
Publication history:
Received on 13 February 2024; revised on 25 March 2024; accepted on 28 March 2024
Abstract:
Background: Sepsis due to ESBL-producing Escherichia coli infection requires innovative management to prevent a higher prevalence of meropenem overuse. The antimicrobial effect of Piper betle leaves ethanolic extract has been previously proven and should be tested on sepsis-induced animal models.
Objectives: This study assesses the antimicrobial effect of Piper betle leaves ethanolic extract towards the bacterial loads and its anti-inflammatory effect on neutrophil infiltration in the lung, kidney, and liver of sepsis-induced mice.
Methods: After sepsis inducement, 3 mg/mL of Piper betle leaves ethanolic extract was administered orally. After the mice died, the bacterial load of the lung, kidney, and liver was measured, while neutrophil infiltration was observed using hematoxylin-eosin staining.
Results: There is no significant decreasing the number of bacterial loads in the treatment group but there is a significant decrease in the number of neutrophils infiltration in the treatment group.
Conclusion: Single dose administration of Piper betle leaves ethanolic extract is sufficient to inhibit neutrophil infiltration significantly.
Keywords:
Piper betle ethanolic extract; ESBL-producing Escherichia coli; sepsis-induced mice; Bacterial load; Neutrophil infiltration
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