Colorimetry-based quantification of protein droplets using "ImageJ"
Faculty of Animal Science, University of Mataram, Jl. Majapahit No. 62 Mataram - NTB, 83125, Indonesia.
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 29(02), 431–434.
Article DOI: 10.30574/gscbps.2024.29.2.0426
Publication history:
Received on 30 September 2024; revised on 18 November 2024; accepted on 21 November 2024
Abstract:
The objective of this study is to analyze protein concentration on a colorimetric basis by utilizing the openly accessible 'ImageJ' software. We herein report the successful measurement of protein concentration on a parafilm surface, following the application of Bradford reagent and subsequent photography with a smartphone, followed by analysis using the ImageJ software. Ovalbumin (OVA) and bovine serum albumin (BSA) were dissolved in phosphate buffered saline (PBS) and used as standards. The results demonstrated a linear correlation between integrated density and the actual weight of the standard, with R² values of 0.9908 for OVA samples in the range of 0-1.5 mg/ml and 0.9781 for BSA in the range of 0.25-1.5 mg/ml. The straightforward nature of the experiments and analyses makes the study we present here an invaluable resource for researchers and students who are constrained by equipment limitations, such as the lack of access to spectrophotometers.
Keywords:
Bovine serum albumin; Ovalbumin; Protein quantification; Smartphone
Full text article in PDF:
Copyright information:
Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
