Determination of the chemical composition and evaluation of the antimicrobial and antioxidant potential of the essential oil from Cryptocarya ovalifolia van der Werff leaves (Lauraceae)

Voara Randriamanantoa-Arisoa 1, Mihajasoa Stella Razanatseheno 2, Lovarintsoa Judicaël Randriamampianina 2, Zara Nomentsoa Razafiarimanga 2, Huchard Paul Bertin Randrianirainy 1, Hanitra Ranjàna Randrianarivo 2, Danielle Aurore Doll Rakoto-Ranoromalala 2, Rianasoambolanoro Rakotosaona 4, Jaconnet Oliva Andrianaivoravelona 3 and Victor Louis Jeannoda 2, *

1 National Center for Industrial and Technological Researches (CNRIT), Chemistry Department, P. O. Box 6294, Antananarivo 101, Madagascar.
2 Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, P. O. Box 906, Antananarivo 101, Madagascar.
3 Higher Polytechnic School of Antananarivo (ESPA), Doctoral School in Sciences and Techniques of Engineering and Innovation, Vontovorona, University of Antananarivo, P. O. Box 1500, Antananarivo 101, Madagascar.
4 National Center for Application of Pharmaceutical Research (CNARP), Chemistry Department, P. O. Box 702, Antananarivo 101, Madagascar.
 
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 26(02), 093–104.
Article DOI: 10.30574/gscbps.2024.26.2.0061
Publication history: 
Received on 01 January 2024; revised on 11 February 2024; accepted on 14 February 2024
 
Abstract: 
The present work aims to evaluate the chemical composition, the antimicrobial and antioxidant properties of the essential oil from Cryptocarya ovalifolia van der Werff (COEO) leaves collected in Mandraka forest. COEO was extracted by steam distillation from fresh leaves, with a yield of 0.7%. COEO appeared as a clear liquid, yellow, with a pleasant odour and relative density of 0.8943 at 20 °C, a refractive index of 1.4746, an optical rotation of – 31°13, an acid index of 0.638 and an ester index of 6.375. A total of 31 constituents accounting for 99.98% of the total essential oil content were identified by gas chromatography/mass spectrometry. The main component was sabinene (30.65%) and the major components (>5%) were limonene (17.33%), methyleugenol (14%) and α-pinene (8.33%). The antibacterial activity was tested on nine pathogenic microorganisms including four Gram positive bacteria, four Gram negative bacteria and one yeast using the disk diffusion and the microdilution assays. COEO was active on 4 strains with inhibition zone diameter (IZD) ranging from 8.7 mm (Bacillus cereus) to 12.7 mm (Clostridium perfringens). Minimum Inhibitory Concentration (MIC) varied from 1.12 to 2.23 mg/ml and Minimum Bactericidal Concentration (MBC) was 8.93 mg/ml for all strains. COEO had bacteriostatic action on Streptococcus pneumoniae, Clostridium perfringens, Bacillus cereus and Candida albicans (MBC/MIC>4) and bactericidal action on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Vibrio fischeri (MBC/MIC≤4). COEO exhibited radical scavenging activity against DPPH with an IC50 of 123.29 mg/ml. By oral route, its LD50 was 1.9/Kg body weight.
 
Keywords: 
Cryptocarya; Lauraceae; Essential oil; Chemical composition; Antimicrobial properties; Antioxidant activity
 
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Copyright information: 
Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0

 

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