Isolation and identification of amylase-producing microorganisms
World Bank African Centre of Excellence in Oilfield Chemicals Research, Department of Microbiology, Department of Chemistry, Department of Chemical Engineering, University of Port Harcourt, Choba, Nigeria.
Research Article
GSC Biological and Pharmaceutical Sciences, 2022, 20(01), 076–082.
Article DOI: 10.30574/gscbps.2022.20.1.0304(2021)
Publication history:
Received on 09 September 2021; revised on 12 October 2021; accepted on 14 October 2021
Abstract:
The research was aimed at isolation and characterization of α-amylase producing microorganisms. Palm wine and soil samples and cassava processing wastewater were obtained from cassava processing sites in Port-Harcourt, River state, Nigeria. A total of 12 isolates comprising of 7 bacterial and 5 fungal strains respectively were obtained. After screening using starch hydrolysis, only 3 bacterial and 2 fungal strain displayed amylase activity. The 3 bacterial isolates, FS-10, PW-4, and PW-1 were identified as Gram-positive rods microscopically while the fungal strains SGA and SGB was identified as a mold through macroscopic examination. Quantification of amylase production showed that FS-10 had the highest amylase activity with 49.88 U/ml closely followed by PW-4 with 49.50 U/ml while the fungus SGB had the least enzyme activity with 10.57 U/ml. Amplification and sequencing of the 16S rRNA gene and the ITS region of the bacterial and fungal isolates respectively was used to identify the isolates. FS-10 was identified as Bacillus flexus (MW522619), PW-4 was identified as Bacillus cereus (OK384566) and SGA as Aspergillus aculeatus (MW577297). The isolates can be used for industrial production of α-amylase.
Keywords:
Hydrolysis; Bacteria; Amylase; Starch; Molecular characterization; Fungi
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