Method development and validation of taraxerol by using high performance liquid chromatography
Dr. Vedprakash Patil Pharmacy College Gevrai Tanda, Paithan Road, Chh. Sambhajinagar, Maharashtra, India.
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 28(03), 180–192.
Article DOI: 10.30574/gscbps.2024.28.3.0332
Publication history:
Received on 08 August 2024; revised on 14 September 2024; accepted on 17 September 2024
Abstract:
Taraxerol is a bioactive compound found in several higher plants that exhibits many specific biological effects, particularly in the field of medicine. Although taraxerol has limited antioxidative efficacy and only modest antibacterial activities, many studies have shown its promise as an anti-plasmodial, antidiabetic, anticancer, anti-inflammatory, and anti-dermatophyte agent. A robust analytical approach for quantifying Taraxerol using High Performance Liquid Chromatography (HPLC) is the main objective of this research endeavor. An analysis of Taraxerol revealed a retention time of 3.519 minutes. Within the concentration range of 20-70 μg/mL, the drug exhibited a linear relationship, with a correlation coefficient of 0.999. The determination of the limit of detection (LOD) and limit of quantitation (LOQ) yielded values of 0.0026 μg/mL and 0.0081 μg/mL each. The accuracy of the approach was considered satisfactory, as the average recovery percentage was within the allowed range of 99.92-100.33%.
Keywords:
RP-HPLC; Method development; Validation; Taraxerol
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