Preliminary phytochemical screening for various secondary metabolites, quantitative and qualitative analysis of Yemeni brown seaweed Sargassum vulgare
1 Department of Pharmacognosy, Pharmacy College, Sana`a University, Sanaa, Yemen.
2 Department of Biology, Faculty of Science, Sana`a University, Sanaa, Yemen.
3 Department of Pharmacy, Faculty of Medical Science, University of Modern Science, Sana`a, Yemen.
4 The Regional Center for Mycology and Biotechnology, Al-Azhar University, 11787, Nasr City, Cairo, Egypt.
Research Article
GSC Biological and Pharmaceutical Sciences, 2022, 20(01), 298–313.
Article DOI: 10.30574/gscbps.2022.20.1.0294
Publication history:
Received on 11 June 2022; revised on 20 July 2022; accepted on 22 July 2022
Abstract:
Sargassum Vulgare was collected from the Red sea coastlines, Al-Hodeida, Yemen, and dried. The dried powder was subjected to consecutive extraction by methanol. The dried methanol extract was subjected to solvent fractionation (n-hexane-chloroform-ethyl acetate and n-butanol solvents). Sargassum vulgare polysaccharides (S.R1P, S.R1M, S.R5s, and S.R5ss) were isolated by precipitation with methanol. [19] The methanol extract and n-hexane fraction were evaluated phytochemically. The physicochemical characteristics and FT-IR analysis of Sargassum vulgare polysaccharides (S.R1P, S.R1M, S.R5s, and S.R5ss) were investigated, the chemical composition of the Sargassum vulgare n-hexane fraction was evaluated by Gas Chromatography-Mass Spectrometry (GC-MS) analysis [8]. Phytochemical investigation revealed that both methanol extract and n-hexane fraction of Sargassum vulgare contained alkaloids, phytosterols, terpenoids, fats, and fixed oils. Moreover, Sargassum vulgare methanol extract exhibited a high amount of carbohydrates (41.33%) mainly polysaccharides [40][41][42]. The physicochemical characteristics and FT-IR analysis of polysaccharides (S.R1P, S.R1M, S.R5s, and S.R5ss) revealed that all the obtained polysaccharides were alginates with traces of sulfate polysaccharides except S.R1M. [30][34][35]
Keywords:
Sargassum Vulgare; Secondary Metabolites; Phytochemical Screening; Physical Tests
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