Kalanchoe Pinnata aqueous extract has the potential to improve haematological profile and fatigue status of mice subjected to Free Swimming Endurance Test
1 Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, P.O. Box 63, Buea (Cameroon).
2 Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé (Cameroon).
Research Article
GSC Biological and Pharmaceutical Sciences, 2021, 17(02), 063–075.
Article DOI: 10.30574/gscbps.2021.17.2.0327
Publication history:
Received on 30 September 2021; revised on 07 November 2021; accepted on 09 November 2021
Abstract:
Introduction: Kalanchoe pinnata extracts have been shown to possess beneficial cardiovascular effects, analgesic and myorelaxant activities. This study aimed at evaluating the haematological and anti-fatigue effects of its aqueous extract.
Materials and methods: This was an in vivo pharmacological experiment, carried out in the Laboratory of Zoology, University of Buea, from January to May 2018. A total of 48 mice were subjected to a 90mins/day-free endurance swimming test for 14 days. Two groups of mice received distilled water and 12% NaCl solution, respectively. Five groups received the plant extract (25, 50, 100 and 200 mg/kg/day) and 200mg/kg/day+NaCl. One positive control group received 5% Vitamin C (1.97mg/kg/day). All administrations were by gavage. Maximum swimming time, glycaemia, lactatemia, uremia, triglyceridemia, haematological indices, tissue glutathion peroxidase, malondialdehyde, glucose and creatine kinase-MB in the heart or gastrocnemius muscle were measured.
Results: K. pinnata (50 and 100mg/kg/day) induced a non-significant increase of the swimming duration, compared to neutral control. However, the 12%NaCl group recorded a significant (P<0.05) increase. In the blood, NaCl induced a decrease of platelets that was significantly reverted by the extract in the 200mg/kg+NaCl group. The extract prevented the increase of the level of CK-MB in NaCl group and decreased the serum triglycerides, glucose, urea nitrogen and Lactate levels. In the tissues, MDA and CK-MB levels significantly increased (P<0.001) in the negative control. These increases were significantly (P<0.001) prevented by K. pinnata (200mg/kg/day).
Conclusions: Significant biochemical variations support the hypothesis that K. pinnata could be used to delay exercise induced fatigue.
Keywords:
Kalanchoe pinnata; Haematological parameters; Biochemical parameters, Exercise; Anti-fatigue; Mice
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