Determination of polyphenolic components by High Performance Liquid Chromatography (HPLC) and evaluation of the antioxidant activity of leaves of Olea europaea L. var. sylvestris (Miller) Lehr
1 Department of Pharmacy, Faculty of Medicine, Badji Mokhtar-Annaba University, Algeria.
2 Laboratory of Development and Control of Pharmaceutical Preparations, Department of Pharmacy, Faculty of Medicine, Badji Mokhtar-Annaba University, Algeria.
Research Article
GSC Biological and Pharmaceutical Sciences, 2022, 20(01), 095–099.
Article DOI: 10.30574/gscbps.2022.20.1.0281
Publication history:
Received on 02 June 2022; revised on 06 July 2022; accepted on 08 July 2022
Abstract:
Olea europaea L. var. sylvestris (Miller) Lehr is an inseparable entity of the Mediterranean people, used in traditional medicine for its many therapeutic virtues.Its leaves are rich in polyphenols.In the present study we wanted to research its polyphenolic components in which it is rich. The method we used is high performance liquid chromatography (HPLC) and evaluation of the antioxidant activity in the methanolic extract of its leaves. For this evaluation we followed the free radical reduction method DPPH (2, 2-diphenyle-1-picryl hydrazyl) by an antioxidant. The results show, the presence of polyphenolic components such ascaffeic acid, chlorogenic acid and rutin, and significant antioxidant activity. Olea europaea L. var. sylvestris (Miller) Lehr is could be placed as an alternative treatment for certain pathologies because it is of value for public health.
Keywords:
Olea europaea L. var. sylvestris; Leaves; Methanolic extract; Polyphenolic components; Antioxidant activity
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