Injectable chitosan- Ulva lactuca polysaccharide hydrogel neutralizes oxidative stress in experimental diabetic neuropathy
1 Department of Marine Biology, Centre of Advanced Study (CAS) in Marine Biology, Annamalai University, Parangipettai - 608 502, Tamil Nadu, India.
2 Department of Biochemistry, Karpagam Academy of Higher Education, deemed to be University, Coimbatore-641021, India.
3 Department of Biochemistry, University of Kerala, Trivandrum-695581, Kerala, India.
Research Article
GSC Biological and Pharmaceutical Sciences, 2022, 21(01), 042–059.
Article DOI: 10.30574/gscbps.2022.21.1.0363
Publication history:
Received on 14 August 2022; revised on 20 September 2022; accepted on 22 September 2022
Abstract:
Polysaccharide –based hydro gels from marine algae are attractive materials for biomedical applications which include polyfunctionality and biodegradability. There were reports of many algae based hydrogels and their applications in diabetic neuropathy. The present study aims to investigate the progressive effects of injectable Chitosan-Ulva lactuca polysaccharide hydrogel (C-UL hydrogel) on experimentally induced diabetic neuropathy in mice. Physicochemical properties of C-UL hydrogel were analyzed by proximate composition, FTIR, SEM, and swelling properties. Diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ, 60 mg/kg). C-UL hydrogel treatment was started 15 days after diabetes induction and continued for 30 days. Serum levels of cholesterol profile and insulin were assessed. In liver, brain, and pancreas tissues, antioxidants superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST) were measured. In the liver, brain and pancreas tissues, C-UL hydrogel attenuated diabetes-induced changes in serum antioxidants and cholesterol excluding insulin. Diabetes-induced dysregulated levels of SOD, CAT, and GST were ameliorated by C-UL hydrogel. Histopathological analysis shows that C-UL hydrogel corrects the altered neuronal changes in diabetic animals. C-UL hydrogel prevents brain damage by regulating the antioxidant pathways in the mice model.
Keywords:
Chitosan- U. lactuca polysaccharide hydrogel; Antioxidants; Diabetes mellitus; Neuropathy; Oxidative stress
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