Amino acid-assisted synthesis and characterization of zero-Valente iron nanoparticles for research on the bio-interactions with yeast
1 State Research Institute Centre for Physical Sciences and Technology, Sauletekio ave. 3, LT-10257, Vilnius, Lithuania.
2 Nature Research Center, Institute of Botany, Akademijos 2, LT-08412 Vilnius, Lithuania.
Research Article
GSC Advanced Engineering and Technology, 2022, 04(01), 001–011.
Article DOI: 10.30574/gscaet.2022.4.1.0043
Publication history:
Received on 10 May 2022; revised on 13 June 2022; accepted on 15 June 2022
Abstract:
The wet synthesis of uniformly sized zero-Valente iron nanoparticles (NPs) for application in nanomedicine and biology is still a challenging because of magnetic interaction, oxidation and corrosion. In this study, various Fe-containing NPs were synthesized using amino acids as their size stabilizing agents to investigate their bio-interactions with Metschnikowia spp. yeast strains. For this, D-glutamic, DL-cysteine, DL-methionine, and taurine amino acids were tested. In this way, NPs in size from several to 40 nm, composed of a-Fe0, a-FeB, Fe2O3 and lepidocrocite, g-FeOOH, were synthesized and characterized using X-ray diffraction and Mössbauer spectroscopy, and HRTEM investigations. The surprising influence of amino acid nature and DL-cysteine concentration on the NP size and composition has been revealed allowing fabrication of quite pure lepidocrocite as well as a-Fe0 NPs containing boron. As-synthesized NPs were cultivated in a YEPD growth media for research on the formation of insoluble red pigment pulcherrimin by several Metschnikowia spp. yeast strains. For comparison, the behavior of M. pulcherrima yeast with metallic iron plate and Fe0 NPs has been visualized.
Keywords:
Zero-Valente Iron; Nanoparticles; Pulcherrimin Yeast; Self-Organization
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Copyright © 2022 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
