Anticholinergic and adrenergic activities of a dichloromethane fraction of a crude ethanol extract of stem bark of Piliostigma reticulatum Horscht D.C (Ceasalpiniaceae)
1 Laboratory of Physiology and Pharmacology, faculty of Environment, University of Jean Lorougnon Guede of Daloa, 02 P.O Box 150 Daloa 02, Côte d'Ivoire.
2 Laboratory of Biotechnology in agro-resources valorization and natural substances, UFR Biological Sciences, University of Korhogo, P.O Box 1328 Korhogo, Korhogo, Côte d'Ivoire.
3 Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, P.O Box KB 52, Korle-Bu, Accra, Ghana.
4 Laboratory of Physiology and Pharmacology, faculty of Natural Sciences, University of Nangui Abrogoua, 01 P.O Box 801 Abidjan 01, Côte d'Ivoire.
Research Article
GSC Advanced Research and Reviews, 2020, 03(01), 001-010.
Article DOI: 10.30574/gscarr.2020.3.1.0012
Publication history:
Received on 22 February 2020; accepted on 07 April 2020
Abstract:
The objective of the present study was to investigate the anticholinergic and adrenergic activities of a dichloromethane fraction of a crude ethanol extract of stem bark of P. reticulatum, on isolated rabbit duodenum contractions. In the first study, the dichloromethane fraction was tested on the isolated rabbit duodenum contractions at increasing concentrations (0.13; 0.26; 0.39 and 0.52 mg/mL) after addition of acetylcholine. In the second study, the dichloromethane fraction was tested on the isolated rabbit duodenum contractions ten minutes after addition of prazosin or propanolol at increasing concentrations (10-4, 10-3 and 10-2 mg/mL). The amplitude and tone induced by acetylcholine were significantly inhibited by the dichloromethane fraction. At a concentration of 0.52 mg/mL, the amplitude induced by the acetylcholine was 0.42±0.05g. The relaxation induced by the dichloromethane fraction was significantly inhibited by prazosin and propanolol as well. At a concentration of 10-2 mg/mL, prazosin and propanolol inhibited the relaxation induced by the dichloromethane fraction at rates of 18.6±2.42 and 17.4±0.7 % respectively. Results showed that the dichloromethane fraction of crude ethanol extract of stem bark of Piliostigma reticulatum exhibited anticholinergic and adrenergic properties that could at least explain and back up its traditional use against diarrhea.
Keywords:
Acetylcholine; Diarrhea; Piliostigma reticulatum; Prazosin; Propranolol.
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