Identification of acetogenin compound, sabadelin from soursop seeds, Annona muricata (Annonaceae)
1 Study Program of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University, Jalan barong Tongkok No.4, Samarinda-75119, Indonesia.
2 College of Industrial and Pharmaceutical Technology (STTIF), Bogor-16151, Indonesia.
3 Research Center for Pharmaceutical Ingredient and Traditional Medicinal, National Research and Innovation Agency (BRIN); KST Soekarno, Cibinong 16911, Indonesia.
4 Faculty of Pharmacy, Pancasila University, Srengseng sawah, Jagakarsa, Jakarta Selatan-12640, Indonesia.
Research Article
GSC Advanced Research and Reviews, 2023, 17(02), 240–246
Article DOI: 10.30574/gscarr.2023.17.2.0446
Publication history:
Received on 12 October 2023; revised on 25 November 2023; accepted on 28 November 2023
Abstract:
Isolation and identification of one chemical compound from the seeds of soursop, Annona muricata (Annonaceae) based on bioactivity-guided fractionation by the Brine Shrimp Lethality Assay (BSLA) have been done. The powder of soursop seed was extracted successively with n-hexane, chloroform, and methanol then the methanol extract was partitioned with ethylacetate water. Isolation and purification of ethylacetate extracts were carried out by column chromatography [(SiO2; i. n-hexane-chloroform = 15:1 ~ 1:1 gave one pure isolatedcompound. Based on Ultra Violet (UV), Infra-Red (IR), Nuclear Magnetic Resonance (NMR), and mass spectra (LC-MS/MS), the isolated Fr-4 was an acetogenin compound, sabadelin which had a weak cytotoxicity LC50 of 487 ppm.
Keywords:
Annona muricata; Acetogenin; Sabadelin; Cytotoxic; Brine Shrimp Lethality Test
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