Involvement of CO2 generated by urease in multiplication of Helicobacter pylori
1 Department of Bacteriology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
2 Department of Gastroenterology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
3 Department of Molecular Bacteriology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
4 Department of Biochemistry, Chiba University Graduate School of Pharmaceutical Sciences, Chiba, Japan.
Research Article
GSC Advanced Research and Reviews, 2021, 07(03), 045–053.
Article DOI: 10.30574/gscarr.2021.7.3.0123
Publication history:
Received on 10 May 2021; revised on 12 June 2021; accepted on 15 June 2021
Abstract:
Helicobacter pylori (H. pylori) urease generates both ammonia (NH3) and carbon dioxide (CO2) from urea. NH3 helps H. pylori to survive in the stomach in part by neutralizing gastric acid. However, the relationship between CO2 and H. pylori is not completed cleared. We examined the effect of CO2 generated by urease on multiplication of H. pylori by using isogenic ureB mutant and ureB complemented strain from H. pylori strain JP26. Wild-type strain survived in the medium supplement with 1mM urea in room air, however, the urease negative strain did not. To discern whether CO2 was incorporated into H. pylori, 14C in bacillus was counted after 6 hours incubation with 14C urea in both acidic and neutral medium. Significant more 14C uptake was detected in wild-type strain compared to ureB mutant strain and this uptake in the wild-type strain was more under acidic condition compared to under neutral condition, but no difference was identified in the mutant strain. These results suggest that CO2 generated by urease plays a role in multiplication of H. pylori.
Keywords:
Helicobacter pylori; Urease; CO2; ureB
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