Phenolic compound and total antioxidant contents of seed extracts from 5 varieties of Vigna subterranea from Chad

Abel MBAIOGAOU 1, *, Michel NAITORMBAIDE 2, Bery Leouro MBAIOSSOUM 3, Severin MBAIHOUGADOBE 1 and Salomon Madjitoloum BETOLOUM 1

1 Laboratory of Research on Natural Substances, Faculty of Pure and Applied Sciences (F.P.A.S.), University of N'Djamena, Chad.
2 Chadian Institute of Agricultural Research for Development (CIARD), Bébédjia, Chad.
3 Laboratory of Modelling, Mathematics, Computer Science, Applications and Simulation, Faculty of Pure and Applied Sciences (F.P.A.S.), University of N'Djamena, Chad.
 
Research Article
GSC Advanced Research and Reviews, 2023, 17(02), 001–009.
Article DOI: 10.30574/gscarr.2023.17.2.0405
Publication history: 
Received on 16 September 2023; revised on 01 November 2023; accepted on 03 November 2023
 
Abstract: 
Vigna Subterranea is a plant whose seeds are widely consumed in Chad. Five seed varieties from the Chadian Institute of Agronomic Research for Development (CIARD) in, Chad. Total polyphenol and total anthocyanin contents were assessed by the Folin-Ciocalteu and pH-differential methods respectively. Total antioxidant levels were determined by DPPH and ABTS. The total polyphenol contents obtained were 11.647 mg GAE/g for B12 seed extract, 8.234 mg GAE/g for B16 seed extract, 0.98450 mg GAE/g for B9 seed extract, 1.625 mg GAE/g for B7 seed extract and 0.230 mg GAE/g for B3 seed extract. The total anthocyanin content of these extracts were 0.0100 mg/g, 0.0098 mg/g, 0.0063 mg/g, 0.0026 mg/g and 0.0013 mg/g for B16, B7, B12, B9 and B3 respectively for the five varieties. Results for antioxidant potency showed that the B12 seed extract contains most total antioxidants. Followed by seed extracts from varieties B16, B7 and B9 varieties. Seed extract from variety B3 contains less antioxidants.
 
Keywords: 
Vigna Subterranea; Total polyphenols; Total anthocyanins; Total antioxidants; Chad.
 
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