Diagnostic advances in sepsis: A comparative analysis of syndromic approaches and molecular biology techniques in clinical bacteremia
Department of Bacteriology and Virology, Avicenna Military Hospital, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakech, Morocco.
Research Article
GSC Advanced Research and Reviews, 2024, 21(02), 082–087.
Article DOI: 10.30574/gscarr.2024.21.2.0417
Publication history:
Received on 23 September 2024; revised on 02 November 2024; accepted on 04 November 2024
Abstract:
Sepsis remains a leading cause of morbidity and mortality. This retrospective study, conducted at Avicenne Military Hospital in Marrakech over ten years, analyzed 3721 blood cultures, identifying 420 cases of bacteremia (11.3% positivity rate). The predominant isolates included Staphylococcus aureus, coagulase-negative staphylococci, Escherichia coli, Klebsiella spp., and Acinetobacter baumannii, with significant resistance patterns observed. Notably, 50% of S. aureus were methicillin-resistant (MRSA), while extended-spectrum beta-lactamase (ESBL) production was found in 30% of E. coli and Klebsiella pneumoniae isolates. The FilmArray multiplex PCR system was utilized to enhance rapid pathogen identification, reducing turnaround time from 72 hours to under 2 hours, and demonstrated high concordance with conventional cultures. The molecular diagnostics identified various resistant strains, including carbapenemase-producing organisms in K. pneumoniae and multidrug-resistant A. baumannii, underscoring the critical need for effective antimicrobial stewardship. Overall, this study emphasizes the evolving epidemiology of bacteremia, the rising antimicrobial resistance, and the utility of rapid molecular diagnostics in guiding timely and appropriate treatment, ultimately aiming to improve patient outcomes in sepsis management.
Keywords:
Sepsis; Antibiotic Resistance; Epidemiology; Film Array; Molecular biology
Full text article in PDF:
Copyright information:
Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
