Isolation and antibiotic susceptibility test of bacteria from healthcare workers and hospital environment

Ahmed H.A.1 * and Alkali I.Y 2

1Department of Pharmacognosy and Ethnomedicine, Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto.
2Department of Pharmacology and Therapeutics Usmanu Danfodiyo University Sokoto
 
Research Article
GSC Biological and Pharmaceutical Sciences, 2018, 04(01), 044–047
Article DOI: 10.30574/gscbps.2018.4.1.0047

 

Publication history: 
Received on 11 June 2018; revised on 29 June 2018; accepted on 02 July 2018
 
Abstract: 
Hospital-acquired infections are one of the most significant adverse events for healthcare institutions increasing morbidity and mortality. The hospital environment is a potential source of infection especially with pathogenic bacteria. The study was aimed at identifying some possible sources of pyogenic bacterial infections in a Private hospital and determining the antibiotic susceptibility. Bacteriological analysis of swabs from hands of health care staff, the floors in the wards and clinics, beds and examination couches in the clinics and wards was carried out. Organisms were identified by Gram stain, catalase and coagulase test oxidase test.Bacillus spp and Streptococcus spp were isolated from the floor, bed and air. Staphylococcus spp was isolated from the hands of health workers. The organisms isolated were resistant to cloxacillin, amoxicillin, and streptomycin but susceptible to ofloxacin, gentamicin and co-trimoxazole.  The findings in this study reveal that the hospital environment and the hands of healthcare workers can serve as a reservoir for antibiotic resistant microorganisms.
Keywords: 
Antibiotic susceptibility; Bacillus spp; Staphylococcus spp; Streptococcus spp
 
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