Assessment of steviol on pro-inflammatory cytokines release in human CD14+ cells
1 Department of Companion Animal Clinical Science, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
2 Department of Preventive and Social Medicine, Faculty of Medicine, Srinakharinwirot University, Nakhonnayok, Thailand.
3 Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand.
Research Article
GSC Biological and Pharmaceutical Sciences, 2018, 04(02), 037–042
Article DOI: 10.30574/gscbps.2018.4.2.0048
Publication history:
Received on 12 June 2018; revised on 23 July 2018; accepted on 25 July 2018
Abstract:
Steviol is a major metabolite of stevioside which is a natural noncaloric sweetener. The aim of this present study was to investigate an inhibitory activity of steviol on pro-inflammatory cytokines release from lipopolysaccharide (LPS)-stimulated human CD14+ cells. CD14+ cells were incubated with different concentrations of steviol in the absence or presence of LPS. TNF-α, IL-1β and IL-6 level were determined by using human enzyme-linked immunosorbent assay (ELISA) kits. Steviol at the concentration of 1-100 µM significantly inhibit (P<0.05) the release of TNF-α (966.0+66.0, 906.3+36.7 and 659.3+52.3 vs 1190.3+75.5 pg/ml) and IL-1β (2834.0+67.2, 2440.0+70.5 and 2181.3+143.3 vs 3226.7+106.6 pg/ml) in a dose dependent manner when compared to LPS-treated CD14+ cells. While the inhibition of IL-6 (P<0.05) was found at 10 µM and 100 µM (2171.7+44.3 and 1902.3+58.0 vs 2580.3+105.2 pg/ml). In conclusion, steviol, a major metabolite of stevioside, possess an anti-inflammatory activity in human CD14+ cell.
Keywords:
Steviol; TNF-α; IL-1β; IL-6; CD14+ cell
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Copyright © 2018 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0.
