Extracellular enzymes and βeta-lactamase screening of bacterial isolates cultured from urine samples of immunocompromised women

Olagoke O. V. 1, 2 *, Alao B. I. 2, Jimoh J. O. 2 and Ladipo T. O.

1 Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
2 Department of Agricultural Development Management, Agricultural and Rural Management Training Institute (ARMTI), Ilorin, Nigeria.
 
Research Article
GSC Biological and Pharmaceutical Sciences, 2018, 04(03), 037–041
Article DOI: 10.30574/gscbps.2018.4.3.0043
Publication history: 
Received on 05 June 2018; revised on 13 August 2018; accepted on 20 August 2018
 
Abstract: 
Enzymes intricate by organisms are used to inactivate and destroy antibiotics. This study determined some virulence factors elaborated by the bacterial isolates cultured from urine samples of HIV seropositive pregnant women that attended antenatal clinic of the Ondo State Specialist Hospital, Akure. Screening for extracellular enzymes and extended spectrum beta lactamase (ESBL) of the isolate was carried out using standard method and disk approximation method respectively. The extracellular enzymes such as lipase, protease and DNase were detected in the three bacterial genera used. ESBL was found in S. aureus and E. coli. The study concluded that most of the bacterial isolates cultured from urine samples of HIV seropositive pregnant women in the study area were found to have these virulence factors.
Keywords: 
Screening; Extracellular enzymes; Bacteria; HIV seropositive
 
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