Effectiveness of selected broad spectrum antibiotics against Pseudomonas species isolated through the use of chloramphenicol
Department of Microbiology, Faculty of Science, Rivers State University, P. M. B. 5080 Port Harcourt, Nigeria.
Research Article
GSC Biological and Pharmaceutical Sciences, 2018, 05(03), 099–105.
Article DOI: 10.30574/gscbps.2018.5.3.0158
Publication history:
Received on 08 December 2018; revised on 20 December 2018; accepted on 24 December 2018
Abstract:
Greenish pigment producing Pseudomonas species were isolated from water samples using nutrient agar supplemented with 50 μg.ml-1 chloramphenicol. Broth cultures of three of the Pseudomonas isolates were prepared for minimum inhibitory concentration (MIC) determination by adjusting their turbidity using sterile normal saline to match the turbidity of McFarland standard No. 1 which is regarded to have a cell density of about 3 × 108 cfu/ml. The MIC of ciprofloxacin, erythromycin, and tetracycline against the three isolates was determined using the agar dilution method. The concentrations of the antibiotics used are 0.625, 1.25, 2.5, 5.0, 10.0, 20.0, 40.0, 80.0, 160.0, 320.0, and 640.0 μg/ml. The results showed that the MIC of ciprofloxacin against the Pseudomonas isolates ranged from 5 – 640 μg/ml; the MIC of tetracycline against the isolates ranged from 5 – 640 μg/ml; and the MIC of erythromycin against the isolates ranged from 40 - 80 μg/ml. It is thus concluded that multidrug resistant species of Pseudomonas can be generated as a result of using chloramphenicol for the isolation of Pseudomonas, and that erythromycin can be effective in treating infections caused by Pseudomonas species that are resistant to ciprofloxacin and tetracycline.
Keywords:
Pseudomonas species; Chloramphenicol; Minimum inhibitory concentration; Broad spectrum antibiotics; Multi-drug resistance
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