Biochemical evaluation of antibacterial activity of short and medium chain fatty acids in broiler

Maksoud Hussein Abdel; Khaid Mohamed Mahfouz; Abd Elmagid Desoky Afaf; Hegazy Yomna; Fotouh Ahmed

doi:10.30574/gscarr.2019.1.1.0007

Authors

Maksoud Hussein Abdel Biochemistry and Clinical Biochemistry Department, Benha University, Egypt.
Khaid Mohamed Mahfouz Biochemistry and Clinical Biochemistry Department, Benha University, Egypt.
Abd Elmagid Desoky Afaf Biochemistry and Clinical Biochemistry Department, Benha University, Egypt.
Hegazy Yomna Biochemistry and Clinical Biochemistry Department, Benha University, Egypt.
Fotouh Ahmed Department of Parasitology and Animal Diseases, National Research Center, Egypt.

DOI:

https://doi.org/10.30574/gscarr.2019.1.1.0007

Keywords:

Organic acids, Short chain fatty acid, Medium chain fatty acid, Antibacterial activity

Abstract

The objective of the present study was to evaluate the antimicrobial effect of short and medium fatty acid chain. Total number of 2000 Cobb broiler chicks (mixed sexes) were commercially purchased from EL Dakahlia poultry company that were 1d old were reared up to 40d of age. Corn and soybean meal based starter and grower diet were supplemented. Chicken were randomly divided in to two main group, 1^st group act as normal control, 2^nd group was add C12( mixed short and medium fatty acid) in drinking water for 3 day each 8 day at 11, 22 and 33 days age. Blood sample were collected before and after taking C12 treatment for biochemical examination. Supplementation of C12 caused decrease in serum level of AST, ALT, glucose, cholesterol, triglyceride, and pro-inflammatory cytokines as IL-6, increase HDL and total protein. Evaluation of antimicrobial activity of C12.

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References

Van Der Wielen PW, Biesterveld S, Notermans S, Hofstra H, Urlings BA and Van Knapen F. (2000). Role of volatile fatty acids in development of the cecal microflora in broiler chickens during growth. Applied and Environmental Microbiology, 66 (6), 2536-40.

Adil S, Banday T, Bhat GA, Mir MS and Rehman M. (2010). Effect of dietary supplementation of organic acids on performance, intestinal histomorphology, and serum biochemistry of broiler chicken.

Saki AA, Harcini RN, Rahmatnejad E and Salary J. (2012). Herbal additives and organic acids as antibiotic alternatives in broiler chickens diet for organic production. Afr. J. Biotechnol, 11, 2139–2145.

Windisch W, Schedle K, Plitzner C and Kroismayr A. (2008). Use of phytogenic products as feed additives for swine and poultry. Journal of Animal Science, 86, 140–148.

Van Immerseel F, J De Buck, I De Smet, F Pasmans, F Haesebrouck and en R Ducatelle. (2004). Interactions of butyric acid and acetic acid treated salmonella with chicken primary cecal epithelial cells in vitro. Avian Dis. 48, 384-391.

Humphrey TJ and Lanning DG. (1988). The vertical transmission of salmonellas and formic acid treatment of chicken feed: A possible strategy for control. Epidemiology and Infection, 100, 43-49.

Kwan YM and Ricke SC. (2005). Induction of acid resistance of Salmonella typhimurium by exposure to short chain fatty acids. Appl. Environ. Microbiol, 64(9), 3458 – 3463.

Odle J. (1997). New insights into the utilization of medium-chain triglycerides by the neonate: Observations from a piglet model. J Nutr, 127, 1061–7.

Jacobi SK and Odle. (2012). Effect of dietary supplementation of organic acids on performance and serum biochemistry of broiler chicken. Natur. Sci, 12(2), 38-45.

Rossi R, Pastorelli G, Cannata S and Corino C. (2010). Recent advances in the use of fatty acids as supplements in pig diets: a review. Anim Feed Sci Technol, 162, 1–11.

Dierick N, Decuypere J, Molly K, Van Beek E and Vanderbeke E. (2003). The combined use of triacylglycerol containing medium-chain fatty acids (MCFAs) and exogenous lipolytic enzymes as an alternative for nutritional antibiotics in piglet nutrition. Nutrition Research Reviews, 16, 193–209.

Huyghebaert G, Ducatelle R and Immerseel FV. (2011). An update on alternatives to antimicrobial growth promoters for broilers. Vet. J, 187, 182-188.

Sato H, Nitana A, Kurosawa T and Oikawa S. (2004). Anticoccidial efficacy of medium-chain triglycerides (MCT) in calves J Vet Med Sci, 66(12), 1583-1585.

Murray. (1984). Report on the symposium “drug effect in clinical chemistry methods for Estimation of ALT. EurJ Clin Chem clin Biochem., 34, 1088-1090.

Burtis A, Carl A, Edward R and Ashwood MD. (1999). Tietz Textbook of clinical chemistry 3rd Edition.

Schettler G and Nussel E. (1975). Colorimetric determination of Total cholesterol, Triglycerides. Arb. Med. Soz. Med. Prav. Med., 10, 25.

Gordon T. (1977). Colorimetric determination of serum HDL cholesterol. Amer. J.Med, 62, 707.

Friedewald WT. (1972). Colorimetric determination of serum LDL cholesterol,Clin. Chem., (18), 499.

Caraway WT and Watts NB. (1987). 3rd ed. Philadephia WB sande-rs, 422-447.

Bancroft JD and Stevens A. (1996). Theory and practice of histological technique.4th edition. Churchill, Livingston, New York, London, San Francisco, Tokyo.

Deepa K, Purushothaman MR, Vasanthakumar P and Sivakumar K. (2017). Serum Biochemical Parameters and Meat Quality Influenced Due to Supplementation of Sodium Butyrate in Broiler Chicken. Int. J. Livestock Res., 7(8), 108-116.

Sarono S. (2003). In vitro probiotic properties of indigenous dadih lactic acid bacteria.Asian- Australian J. Ani. Sci., 16(5), 726-731.

Klaver FA and R Van der Meer. (1993). The assumed assimilation of cholesterol by Lactobacillus and Bifidobac0terium bifidum is due to their bile salt deconjugating activity. Appl. Enviro. Microbio, l. 59(4), 1120-1124.

Sheikh A, Tufail B, Gulam AB, Masood SM and Manzoor R. (2010). Effect of dietary supplementation of organic acid on performance, intestinal histomorphology, and serum biochemistry of broiler chicken. Vet Med Int, 479485.