Anticholinergic and adrenergic activities of a dichloromethane fraction of a crude ethanol extract of stem bark of Piliostigma reticulatum Horscht D.C (Ceasalpiniaceae)

Kassim Dosso; Dago Serge David Attemene; Abiba Ouattara Gboko; Benoit Banga N’guessan; Angoue Paul Yapo

doi:10.30574/gscarr.2020.3.1.0012

Authors

Kassim Dosso Laboratory of Physiology and Pharmacology, faculty of Environment, University of Jean Lorougnon Guede of Daloa, 02 P.O Box 150 Daloa 02, Côte d'Ivoire.
Dago Serge David Attemene Laboratory of Physiology and Pharmacology, faculty of Environment, University of Jean Lorougnon Guede of Daloa, 02 P.O Box 150 Daloa 02, Côte d'Ivoire.
Abiba Ouattara Gboko Laboratory of Biotechnology in agro-resources valorization and natural substances, UFR Biological Sciences, University of Korhogo, P.O Box 1328 Korhogo, Korhogo, Côte d'Ivoire.
Benoit Banga N’guessan Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, P.O Box KB 52, Korle-Bu, Accra, Ghana.
Angoue Paul Yapo Laboratory of Physiology and Pharmacology, faculty of Natural Sciences, University of Nangui Abrogoua, 01 P.O Box 801 Abidjan 01, Côte d'Ivoire.

DOI:

https://doi.org/10.30574/gscarr.2020.3.1.0012

Keywords:

Acetylcholine, Diarrhea, Piliostigma reticulatum, Prazosin, Propranolol

Abstract

The objective of the present study was to investigate the anticholinergic and adrenergic activities of a dichloromethane fraction of a crude ethanol extract of stem bark of P. reticulatum, on isolated rabbit duodenum contractions. In the first study, the dichloromethane fraction was tested on the isolated rabbit duodenum contractions at increasing concentrations (0.13; 0.26; 0.39 and 0.52 mg/mL) after addition of acetylcholine. In the second study, the dichloromethane fraction was tested on the isolated rabbit duodenum contractions ten minutes after addition of prazosin or propanolol at increasing concentrations (10^-4, 10^-3 and 10^-2 mg/mL). The amplitude and tone induced by acetylcholine were significantly inhibited by the dichloromethane fraction. At a concentration of 0.52 mg/mL, the amplitude induced by the acetylcholine was 0.42 ± 0.05g. The relaxation induced by the dichloromethane fraction was significantly inhibited by prazosin and propanolol as well. At a concentration of 10^-2 mg/mL, prazosin and propanolol inhibited the relaxation induced by the dichloromethane fraction at rates of 18.6 ± 2.42 and 17.4 ± 0.7 % respectively. Results showed that the dichloromethane fraction of crude ethanol extract of stem bark of Piliostigma reticulatum exhibited anticholinergic and adrenergic properties that could at least explain and back up its traditional use against diarrhea.

Metrics

Metrics Loading ...

References

Hasler WL. (2003). Pharmacotherapy for intestinal motor and sensory disorders. Gastroenterology Clinical of North America, 32, 707-732.

Farhadi A, Bruninga K, Fields J and Keshavarzian A. (2001). Irritable bowel syndrome: an update on therapeutic modalities. Expert Opinion Investigation Drugs, 10, 1211-1222.

Pasricha PJ. (2001). Prokinetic agents, antiemetics and agents used in irritable bowel syndrome. In: Hardman JG, Limbird LE, Goodman Gilman A. (Eds.), Goodman & Gilman’s the Pharmacological Basis of Therapeutics. McGraw Hill, New York, 1021–1036.

Valiollah H, Sadraei H, Ghannadi AR and Mohseni M. (2000). Antispasmodic and anti-diarrhoeal effect of Satureja hortensis L. essential oil. Journal of Ethnopharmacology, 71, 187-192.

Ragone MI, Sella M, Conforti P, Volonté MG and Consolini AE. (2007). The spasmolytic effect of Aloysia citriodora Palace (Souch American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums. Journalof Ethnopharmacology, 133, 258-266.

Borrelli F, Borbone N, Capasso R, Montesanoc D, De Marino S, Aviello G, Aprea G, Masone S and Izzo AA. (2009). Potent relaxant effect of a Celastrus paniculatus extract in the rat and human ileum. Journalof Ethnopharmacology, 122, 434-438.

Corallo MA, Founbge S and Declume C. (1988). Etude expérimentale de l'activité utérotonique de Bridelia atroviridis et Phyllanthus discoideus (Euphorbiacées). Mise en évidence d'un effet au niveau des récepteurs α-adrénergiques. Annales Pharmaceutiques Francaises, 46, 171-177.

Koudou J, Aklikok K, Gbeassor M and Bessiere JM. (2001). Composition chimique et activité anticontracturante de l'huile essentielle de Monodora myristica gaertn de la république centrafricaine. Pharmacopée and Medecine Traditionnelle Africaine, 11, 59-67.

Gilani AH, Shah AJ and Yaeesh S. (2007). Presence of cholinergic and calcium antagonist constituents in Saussurea lappa explains its use in constipation and spasm. Phytotherapy Research, 21, 544-1544.

Salawu SO, Tijani AY, Obidike IC and Chindo BA. (2009). Anti-ulcerogenic properties of methanolic root extract of Piliostigma reticulatum (DC) Hoechst (Syn. Bauhinia reticulate DC) -Leguminosae in rats. African Journal of Pharmacy and Pharmacology, 3(5), 252-258.

Arbonier M. (2002). Arbres, Arbustes et Lianes des zones sèches de l’Afrique de l’Ouest. CIRAD, 2nd edn. MNHN, 2574.

Aderogba MA, Okoh EK, Ogundaini AO and Abegaz BM. (2003). C-methylquercetin-3, 3’, 4’-trimethyl ether from the leaves extract of Piliostigma reticulatum. Nigerian Journal of Natural products Medicine, 7, 37-8.

Salawu SO, Tijani AY, Obidike IC and Chindo BA. (2007). Evaluation of anti-diarrhoeal properties of methanolic Root extract of Piliostigma reticulatum in rats. Journal of Phytomedicine and Therapeutics, 12.

Yelemou B, Bationo B, Yameogo G and Millogo-Rasolodimby J. (2007). Bois et Forêts des tropiques. ISSN 0006-579X, Coden, 291, 55-66.

Dosso K, N’guessan BB, Gnangoran BN and Yapo AP. (2014). Acute toxicity effects of dichloromethane fraction of ethanol extract of stem bark of Piliostigma reticulatum on rats. African Journal of Plant Science, 8(8), 405-409.

Dosso K, N’guessan BB, Bidie AP, Gnangoran BN, Méité S, N’guessan D, Yapo AP and Ehilé EE. (2012). Antidiarrhoeal activity of an ethanol extract of the stem bark of Piliostigma reticulatum (Caesalpiniaceae) in rats. Afrcan Journal of Traditional Complementary and Alternative Medicine, 9(2), 242-249.

Dosso K, N’guessan BB, Amoateng P and Gnangoran BN. (2012). Anti-secretory effects of a dichloromethane fraction of stem bark of Piliostigma reticulatum (Cesalpiniaceae). Journal of Medical and Biomedical Sciences, 1(3), 13-20.

N’guessan BB, Dosso K, Gnangoran BN, Amoateng B, Asiedu-Gyekye IJ and Yapo AP. (2015). Antibacterial and antispasmodic activities of a dichloromethane fraction of an ethanol extract of stem bark of Piliostigma reticulatum. Journal of Pharmacy and BioAllied Science, 7(2), 128-135.

Kone M, Yao KE, Gbogbo M, N’dri NM and Yapo AP. (2016). Effects of the ethanol extract of Pilistigma reticulatum DC (Horscht) stem bark on biochemical parameters of albino rat wistar. Scholars Academic Journal of Biosciences, 4(1), 11-17.

Harborn JB. (1984). Phytochemical methods. In: A Guide to ModernTechniques of Plant Analysis. Chapman and Hall, 2nd edn, London, 4-7.

Samsam-Shariat SH. (1992). Qualitative and Quantitative Evaluation of the Active Constituents and Control Methods for Medical Plants. Mani Publications, Isfahan (in Persian), 23-30.

Mitjans M, Garcia L, Marrero E and Vinardell MP. (2008). Study of ligmed-A, an antidiarrheal drug based on liguin, on rat small intestine enzyme activity and morphometry. Journal of Veterinary Pharmacology and Therapeutics, 24, 349-351.

Magnus R. (1904). Persuche am überbunden Dünndarm Yon Saugetieren. Archive of Ges Physiology, 102123.

Perry WLM. (1968). Pharmacological Experiments on Isolated Preparations. E & S Livingstone Ltd. Edinburgh, 8.

Tona L, Kambu K, Mesia K, Cimanga K, Bruyne T, Pieters L, Totté J and Vlietinck AJ. (1999). Biological screening of traditional preparations from some medicinal plants used as antidiarrhoeal in Kinshasa, Congo. Phytomedicine, 6, 59-66.

Fatou BS, Mamadou S, Doudou D, Modou OK, Abdoulaye B, Bocar S, Lamine G, Aminata SD, Abdoulaye S, Ramaroson A and Fallou C. (2010). In vitro modulation of tracheal smooth muscle reactivity by extracts of some Senegalese medicinal plants. Journal of Medecine Plants Research, 4, 13-18.

Traoré F, Bahi C, Soro YT and Koné PP. (2004). Mise en évidence et caractérisation pharmacologique des principes actifs d’un extrait aqueux de Bridelia ferruginea (Euphorbiaceae). Revue de la Médecine et la Pharmacopée Africaine, 18.

Békro Y, Békro J, Boua BB, Tra Bi F and Ehile EE. (2007). Etude ethnobotanique et screening phytochimique de Caesalpinia benthamiana (Baill.) Herend et Zarrucchi (Caesalpiniaceae). Sciences de la Nature, 4(2), 217-225.

Gilani A, Shaheen F, Christopoulos A and Mitchelson F. (1997). Interaction of ebeinone, an alkaloid from Fritillaria imperialis, at two muscarinic acetylcholine receptor subtypes. Life Science, 60, 535-544.

Guata Y, Aminata S, Amadou M and Babacar F. (2004). Myorelaxant and antispasmodic effects of the aqueous extract of Mitragyna inermis barks on Wistar rat ileum. Fitoterapia, 75, 447-450.

Bolton TB. (1979). Mechanism of action transmitters and other substances on smooth muscle. Physiology Revue, 59, 607-718.

Mollard P, Mironneau J, Amedee T and Mironneau C. (1984). Action potentiel and Ca2+ inward current in isolated pregnant rat myometrial cell. In “Regulation calcique dans les muscles lisses”. Colloque INSERM/CNRS, Editions INSERM, 124, 53-54.

Aziz M, Tab N, Karim A, Mekhfi H, Bnouham M, Ziyyat A and Legssyer A. (2006). Relaxant effect of aqueous extract of Cistus ladaniferus on rodent intestinal contractions. Fitoterapia, 77, 425-428.

Hejazian SH, Morowatisharifabad M and Mahdavi SM. (2007). Relaxant Effect of Carum copticum on intestinal motility in ileum of rat. World Journal of Zoology, 2, 15-18.

Bigovic D, Brankovic S, Kitic D, Radenkovic M, Jankovic T, Savikin K and Zivanovic S. (2010). Relaxant effects of the ethanol extract of Helichrysum plicatum (Asteraceae) on isolated rat ileum contractions. Molecules, 15, 3391-3401.

Triggle CR. (1985). Adrenergic interactions in smooth muscle contractility. In « Calcium and Contractility ». Ed. By A.K. Grover and E.E. Daniel, The HUMANA press, 37- 60.

Guimaraes S and Moura D. (2001). Vascular adrenoreceptors, an update. Pharmacology Revue, 53, 319-356.

Tripathi K. (1994). Essentials of Medical Pharmacology. Jaypee Brothers, New Delhi, 779.

Venkatesan N, Thiyagarajan V, Narayanan S, Arul A, Raja S, Kumar SGV, Rajarajan T and Perianayagam JB. (2005). Antidiarrheal potential of Asparagus racemous wild root extracts in laboratoire animals. Journal of Pharmacy and Pharmaceutical Sciences, 8, 39.

Capasso F, Pinto A, Mascolo N, Autore G and Franco MP. (1988). Effects of flavonoids on PGE2-and LT4-induced contractions on the guinea pig isolated ileum. Pharmacology Research Commun, 20(1), 201-202.