Effect of Vernonia amygdalina on phospholipase activity from Naja Mossambica (Cobra)

Abdullahi Abdulkakdir; Omeremime Elizabeth Dania; Bala Alkali Mohammed; Yahaya Abubakar Mohammed; Maimuna Bello Umar; Rahinat Garba

doi:10.30574/gscarr.2021.6.3.0032

Authors

Abdullahi Abdulkakdir Department of Biochemistry, School of Life Sciences, Federal University of Technology, Minna, Nigeria.
Omeremime Elizabeth Dania Department of Biochemistry, Covenant University, Ota, Ogun State, Nigeria.
Bala Alkali Mohammed Department of Biochemistry, School of Life Sciences, Federal University of Technology, Minna, Nigeria.
Yahaya Abubakar Mohammed Department of Biochemistry, School of Life Sciences, Federal University of Technology, Minna, Nigeria.
Maimuna Bello Umar Department of Biochemistry, School of Life Sciences, Federal University of Technology, Minna, Nigeria.
Rahinat Garba Department of Biochemistry, School of Life Sciences, Federal University of Technology, Minna, Nigeria.

DOI:

https://doi.org/10.30574/gscarr.2021.6.3.0032

Keywords:

Activity, inhibition, Vernonia amygdalina, Naja mossambica

Abstract

Background: Phospholipases are one of the numerous enzymes found in the Naja mossambica venom. They play a major role in snakebite envenomation, and also responsible for the hydrolysis of a phospholipid, disrupting the membrane integrity. In this study, we evaluated the effect of Vernonia amygdalina on Phospholipase activity from Naja mossambica (Cobra) Results: Partially purified phospholipase had maximal velocity (V_max) and Michaelis Menten constant (K_m) of 7.6 × 10^-5mol/min and 1.7mg/ml, while the crude phospholipase had V_max and K_m of 9.4 × 10^-5mol/min and 2.5mg/ml respectively. Inhibition study of aqueous extracts of Vernonia amygdalina leaf shows that the extract is a potent inhibitor of crude phospholipase in a dose-dependent pattern. The different doses of extract 15 %, 10 % and 5% produced percentage inhibition of 74.04 %, 78.6 % and 86.63% respectively. The kinetic binding constant (K_i) values of crude phospholipase for different concentrations of extracts 5%, 10% and 15% were 0.21mg/ml, 0.29mg/ml and 0.39mg/ml, while the partially purified phospholipase for different concentrations of extracts 5%, 10% and 15% were were 0.48mg/ml, 0.42mg/ml and 0.41mg/ml respectively. It can be deduced from the results that the extract inhibits the phospholipase activity in an uncompetitive manner. Conclusion: Aqueous extract of Vernonia amygdalina leaves may contain some bioactive agents that could serve as potent inhibitors of phospholipase from Naja mossambica venom.

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References

Chippaux JP. Snake-bites: Appraisal of the global situation. Bulletin WHO. 1998; 76: 515- 524.

Warrell DA. Clinical features of envenoming of snake bites. In: Bon C, Goyffon M,editor. Envenoming and their treatments. Lyon, Fond. Marcel Merieux. 1996; 63-76.

Sakai A. Clinical features of envenomation by the snake, Yamakagashi Rhabdophistigrinus. Chudokukenkyu. 2007; 20 (3): 235-243.

Six DA, Dennis EA. The expanding superfamily of phospholipase A2 enzymes: classification and characterization. Biochim Biophys Acta. 2000; 1488 (1-2): 1-19.

Kini RM. Excitement ahead: structure, function and mechanism of snake venom phospholipase A2 enzymes. Toxicon. 2003; 42 (8): 827-840.

Sajevic T, Leonardi A, Križaj I, et al. Haemostatically active proteins in snake venoms. Toxicon. 2011; 57: 627-645.

Trusevych EH, Macnaughton WK. Proteases and their receptors as mediators of inflammationassociated colon cancer. Curr Pharm Des. 2015; 21: 2983-2992.

Pozzi N, Chen Z, Di Cera E. How the linker connecting the two kringles influences activation and conformational plasticity of prothrombin. J Biol Chem. 2016; 291: 6071-6082.

Ramanadham S, Hsu FF, Zhang S, Jin C, Bohrer A, Song H, Bao S, Ma Z, Turk J, et al. Apoptosis of insulin-secreting cells induced by endoplasmic reticulum stress is amplified by overexpression of group VIA calcium-independent phospholipase A2 (iPLA2 beta) and suppressed by inhibition of iPLA2 beta. Biochemistry. 2004; 3: 918-930.

Masuda S, Murakami M, Komiyama K, Ishihara M, Ishikawa Y, Ishii T, Kudo I, et al. Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells. FEBS Journal. 2005; 272: 655-672.

Cechetti S, Spadaro F, Gessani S, Podo F, Fantuzzi L, et al. Phospholipases: at the crossroads of the immune system and the pathogenesis of HIV-1 infection. JLB. 2017; 101: 53-75.

Ogundipe, O. Health bitter leaf extract may prevent or delay breast cancer and diabetes. Conference paper. 2005.

Marcia DRD, Ariane GS. Anatomical characters of the medicinal leaf and stem of Gymnanthemum amygdalina (Debile), Sch.Bip. ex Walp. (Asteracea). BJPS. 2013; 49: 719–726.

Bonsi MLK, Osuji PO, Tuah AK, Umunna, NN, et al. Vernonia amygdalina as a supplement to teff straw (Eragrostis tef) fed to Ethiopia Menz, sheep. Agrofor. Syst. 1995; 31: 229–241.

Godwin OI, Wieslaw O, Maria J, et al. Stanislaw, B.; Michael, F.; Adetunde, A.F. Flavonoids from Vernonia amygdalina and their antioxidant activities. J. Agric. Food Chem. 1994; 42: 2445–2448.

Ijeh I.I, Ejike CECC. Current perspectives on the medicinal potential of Vernonia amygdalina. J. Med. Plant. Res. 2011; 5: 1051–1061.

Iwalela, EO, Iwalela, OJ, Adeboye JO, et al. Analgesic, antipyretic, anti-inflammatory effect of methanol, chloroform, and ether extracts of Vernonia amygdalina leaf. J. Ethnopharm. 2003; 86: 229–234.

Dole VP. A relation between non esterified-fatty acids in plasma and the metabolisms of glucose. J Clin Invest. 1956; 35 (2): 150-154.

Robert KM., Daryl KG, Victor WR, et al. Kinetic analysis distinguishes competitive from non-competitive inhibition. Harper’s illustrated biochemistry.27th edition. McGraw Hill LANGE. 2006; 68-71.

Okonogi TZ, Hatton A, Ogiso Mitsui S, et al. Detoxification by permission tannin of snake venom and bacterial toxins. Toxicon. 1979; 17: 524-527.

Abubakar MS, Sule MI, Pateh, UU, AbdulRahman AK, Haruna BM, Jahun BM et al. In vitro snake detoxifying action of leaf extract of Guiera senegalensis. J of Ethnopharm. 2000; 69: 253-257.

Akindahunsi AA, Salawu SO. Phytochemical screening and nutrient-anti-nutrient composition of selected tropical leafy vegetables. African Journal of Biotechnology. 2004; 14: 497-501.

Haslam E. Natural polyphenols (Vegetable tannins) as drugs: Possible modes of action. J Nat Prod. 1996; 59(2): 205-215.

Bethwell OO, Daniel PK. Kenyan medicinal plants used as antivenin: a comparison of plant usage. J of Ethnobiol and Ethnomed. 2006; 2: 7.

Walter FG, Bilden EF, Gibly RL, et al. Envenomations. Critical Care Clinicals. 15: 353-386.

Kini RM. Structure-function relationships and mechanism of anticoagulant Phospholipase A2 enzymes from snake venoms. Toxicon. 2005; 45: 1147-1161.

Lindahl M, Tagesson CF. lavonoids as phospholipase A2 inhibitors: importance of their structure for selective inhibition of group II phospholipase A2. Inflammation. 1997; 21(3): 347-356.