Gas chromatography-Mass spectrometry (GC-MS) analysis of bioactive components present in grape citrus peel in Nigeria

Israel Chukwuemeka  Eneke; Eka Bassey  Essien; Matthew Owhonda  Wegu

doi:10.30574/gscarr.2021.8.1.0152

Authors

Israel Chukwuemeka Eneke Department of Biochemistry, University of Port Harcourt, P.M.B. 5323 East-West Road, Choba, Rivers State, Nigeria
Eka Bassey Essien Department of Biochemistry, University of Port Harcourt, P.M.B. 5323 East-West Road, Choba, Rivers State, Nigeria
Matthew Owhonda Wegu Department of Biochemistry, University of Port Harcourt, P.M.B. 5323 East-West Road, Choba, Rivers State, Nigeria

DOI:

https://doi.org/10.30574/gscarr.2021.8.1.0152

Keywords:

Citrus peels, Bioactive, Gas chromatography, Mass spectrometry

Abstract

Grapefruit (Citrus paradisi) is an important cultivar of the Citrus genus which contains a number of nutrients beneficial to human health. Grapefruit peels are usually thrown away in this part of Nigeria. The research work investigated the bioactive components present in an indigenous citrus peel, grape (Citrus paradisi). Grape fruits were purchased from the fruits garden market in D-Line, Port-Harcourt metropolis and washed with ionized water and allowed to shade dry. The peel of the fruits were separated and subjected to cold extraction using 95% ethanol. The extracts obtained were further extracted in dichloromethane and subjected to GC/MS analysis for characterization of various bioactive components. The gas chromatographic model: 789A (GC) analysis was performed on an agilent technologies interfaced with mass selective detector model: 5975(MSD). The results revealed 25 bioactive components in grape peel with n-Hexadecanoic acid showing the highest concentration of 20.36% and retention time of 18.522min. Nootkatone was the lowest component in the grape peel with concentration 0.74% and retention time of 16.459min. Results shows that grapefruit (Citrus paradisi) has considerable potential as a source of natural bioactive components with different retention times. These fruits residues which otherwise regarded as waste hold promising potentials for medicinal therapy and value added food supplements.

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