Effect of coconut oil on Anopheles gambiae sensu lato (Diptera: Culicidae) larvae tolerance in malaria vector control in Dogbo district in south-western Benin, West Africa

Nazaire  Aïzoun; Arlette  Adjatin; Géorcelin  Alowanou

doi:10.30574/gscarr.2021.9.2.0221

Authors

Nazaire Aïzoun Laboratory of Pluridisciplinary Researches of Technical Teaching (LaRPET), Normal High School of Technical Teaching (ENSET) of Lokossa, National University of Sciences, Technologies, Engineering and Mathematics (UNSTIM) of Abomey, P. O. Box 133 Lokossa, Cotonou, Benin.
Arlette Adjatin Laboratory of Biochemistry, Food and Medicinal Formulations (LaBFAM), National High School of Applied Biosciences and Biotechnologies (ENSBBA) of Dassa-Zoumè, National University of Sciences, Technologies, Engineering and Mathematics (UNSTIM) of Abomey.
Géorcelin Alowanou Laboratory of Ethnopharmacology and Animal Health (LESA), Faculty of Agronomic Sciences, University of Abomey-Calavi (UAC), Cotonou, Bénin.

DOI:

https://doi.org/10.30574/gscarr.2021.9.2.0221

Keywords:

Coconut oil, Siphonal respiration, Malaria vector control, Benin

Abstract

The use of chemical insecticides causes important damages to environment and human health and there is a need to search for alternative solutions. This study aims to investigate on the effect of coconut oil on Anopheles gambiae sensu lato larvae tolerance in malaria vector control in Dogbo district in south-western Benin, West Africa. Larvae of Anopheles gambiae s.l. mosquitoes were collected from breeding sites using the dipping method in May 2020 during the rainy season in Dogbo district. A batch of 25 larvae of fourth instar were exposed to a mixture of coconut oil with distilled water saturated with oxygen containing in each of five glass jars or test cups of same dimensions contained each 48 ml distilled water saturated with oxygen plus 2 ml of coconut oil and one control jar containing no trace of coconut oil. Larval mortality was recorded after 24 hours, 48 hours and 72hours exposure. The results show that the use of coconut oil causes full-grown Anopheles larvae to die by suffocation. After the application of this mixture, the larvae of four instars cannot breathe. The use of coconut oil is effective method for disturbing the siphonal respiration of mosquito larvae. Coconut oil is effective method for mosquito larvae control.

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