Genetic polymorphism of opioid receptors and use of opioid substances by high school students of Cotonou and Parakou (Benin)

Arnaud Coffi  AGBANLINSOU; Razack  OSSENI; Marius O  ADJAGBA; Ferdinand  ADOUNPKE; Odile A  KOUGBLENOU; Assad A BIO  SYA; Adolphe  KPATCHAVI; Anatole  LALEYE

doi:10.30574/gscarr.2021.9.3.0303

Authors

Arnaud Coffi AGBANLINSOU Laboratory of Histology, Reproductive Biology, Cytogenetics and Medical Genetics (LHBRCGM)/Faculty of Health Sciences of Cotonou/University of Abomey-Calavi, 01BP 188; Benin
Razack OSSENI Laboratory of Histology, Reproductive Biology, Cytogenetics and Medical Genetics (LHBRCGM)/Faculty of Health Sciences of Cotonou/University of Abomey-Calavi, 01BP 188; Benin
Marius O ADJAGBA Laboratory of Histology, Reproductive Biology, Cytogenetics and Medical Genetics (LHBRCGM)/Faculty of Health Sciences of Cotonou/University of Abomey-Calavi, 01BP 188; Benin
Ferdinand ADOUNPKE National Laboratory of Narcotics and Toxicology (LNST) - Beninese Centre for Scientific Research and Innovation (CBRSI)/University of Abomey-Calavi, Benin.
Odile A KOUGBLENOU Applied Medical Anthropology Laboratory (LAMA) /University of Abomey-Calavi, Benin, 01BP 188 ; Benin.
Assad A BIO SYA National Laboratory of Narcotics and Toxicology (LNST) - Beninese Centre for Scientific Research and Innovation (CBRSI)/University of Abomey-Calavi, Benin.
Adolphe KPATCHAVI Applied Medical Anthropology Laboratory (LAMA) /University of Abomey-Calavi, Benin, 01BP 188 ; Benin.
Anatole LALEYE Laboratory of Histology, Reproductive Biology, Cytogenetics and Medical Genetics (LHBRCGM)/Faculty of Health Sciences of Cotonou/University of Abomey-Calavi, 01BP 188; Benin

DOI:

https://doi.org/10.30574/gscarr.2021.9.3.0303

Keywords:

Receptors, Opioid, Polymorphism, Teenagers, Young adults

Abstract

In Benin, schools become sometimes the hub for dealing and consuming psychoactive substances; opioids in particular. The objectives of this study are to identify the risk factors related to the use of opioids and investigate the genetic polymorphism of mu and delta opioid receptors of teenagers and young adults who consume opioids in schools. To accomplish this, 453 students participated in this study; R diversity 3.6.1 software in the RStudio environment was used to identify students who experience opioids through ASSIST V3.0 scoring. SNPs A118G on the OPRM gene (µ) and T921C on the OPRD gene (d), were searched by PCR on DNA extracts from peripheral blood of individuals. We identified 54 regular opioid users and 399 non users. This experience begins for most with the consumption of alcohol and tobacco and is facilitated by the proximity of marshlands, kiosks, and pubs near high schools and colleges. The aggressive advertisement combined with relative socio-cultural tolerance just worsen this behavioral deviance. We found no difference in the SNP frequencies of the OPRM (µ) and OPRD (d) genes between students opioids consumers and non-consumers.

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