Molecular diagnosis of SARS-CoV-2: A validation of saliva samples

Natalia Verissimo Rodrigues da Silva 1, Samyra Stéphane Neves Pereira 1, Karine Frehner Kavalco 1, Fabiano Bezerra Menegídio 2, Luanda Medeiros-Santana 1, Liliane Evangelista Visôtto 1 and Rubens Pasa 1, *

1 Laboratory of Molecular Diagnostics, Federal University of Viçosa, campus Rio Paranaíba. Road MG 230 Km 7, LAE Building, room 237, Zip code 38810-000, Rio Paranaíba – MG, Brazil.
2 Center of Biotechnology, University of Mogi das Cruzes, Av. Dr. Cândido X. de Almeida e Souza, 200 - Centro Cívico, Mogi das Cruzes, SP, 08780-911, Brazil.
 
Research Article
GSC Advanced Research and Reviews, 2022, 10(02), 001–003.
Article DOI: 10.30574/gscarr.2022.10.2.0037
Publication history: 
Received on 24 December 2021; revised on 27 January 2022; accepted on 29 January 2022
 
Abstract: 
Nasopharyngeal swabs are the most used in sample collecting for covid-19 tests in SARS-CoV-2 molecular diagnosis. However, this sampling method presents some disadvantages, since, in addition to being dependent on imported materials, it is invasive, causes discomfort in patients, and presents the risk of contamination for the medical collection team. This study aimed at validating saliva samples to obtain viral RNA to be used in the molecular diagnostic test for SARS-CoV-2 using the RT-qPCR technique. Results presented 93.44% concordance in comparison to nasopharyngeal swabs sampling. Therefore, saliva samples used in SARS-CoV-2 RT-qPCR detection tests presented consistent results.
 
Keywords: 
SARS-CoV-2; RT-qPCR; Covid-19; Nasopharyngeal swabs (NPS)
 
Full text article in PDF: 
 
Copyright information: 
Copyright © 2022 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0

 

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