Determination of the fatty-acid composition of four native microalgae species

Oya Irmak Şahin; Arzu Akpınar-Bayizit

doi:10.30574/gscarr.2020.4.1.0053

Authors

Oya Irmak Şahin Chemical Engineering Department, Faculty of Engineering, Yalova University, Yalova, Turkey.
Arzu Akpınar-Bayizit Food Engineering Department, Faculty of Agriculture, Uludag University, Bursa, Turkey.

DOI:

https://doi.org/10.30574/gscarr.2020.4.1.0053

Keywords:

Ankistrodesmus, Chlorella, Chroococcus, Oscillatoria, Fatty acid, Microalgae

Abstract

In this study, two green (Chlorella sp. and Ankistrodesmus sp.) and two blue-green (Oscillatoria sp. and Chroococcus sp.) species of microalgae, which were obtained from a native microalgae culture collection from Turkey, were cultivated and investigated via capillary GC to determine their fatty-acid profiles. Growth media were chosen with consideration of the biological properties of each algae species. Our results, as well as other published data, show that fatty-acid composition offers discriminating features that allow the chemotaxonomic classification of these algae. Fatty-acid profiles and lipid contents in microalgal biomass varied between species. The microalgae containing the highest proportion of lipid in its biomass was in Chroococcus sp. (1.72%), while that containing the lowest was Ankistrodesmus sp. (0.14%). The major fatty acids in Chlorella sp. were C18:0 (26.70%), C18:1 (23.48 ± 0.0005%) and C16:0 (21.05%). C18:1 was the major fatty acid in the other three species of microalgae: Ankistrodesmus sp. (36.83 ± 0.0007%), Oscillatoria sp. (38.51%) and Chroococcus sp. (47.96%). EPA, a nutritionally important polyunsaturated fatty acid, was not observed in Chlorella sp. but was detected in Ankistrodesmus sp., Oscillatoria sp. and Chroococcus sp. at proportions of 0.35%, 0.30% and 0.62%, respectively. In addition, another essential PUFA, DHA, was observed in all microalgae species in varying amounts.

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References

Bordoni A, Di Nunzio M, Danesi F and Biagi PL. (2006). Polyunsaturated fatty acids: From diet to binding to ppars and other nuclear receptors. Genes & Nutrition, 1, 95–106.

Schuchardt JP, Huss M, Stauss-Grabo M and Hahn A. (2010). Significance of long-chain polyunsaturated fatty acids (PUFAs) for the development and behaviour of children. European Journal of Pediatrics, 169, 149–164.

Jump DB. (2002).The Biochemistry of n- 3 Polyunsaturated Fatty Acids Journal of Biological Chemistry, 277, 8755–8758.

Shahidi F and Wanasundara UN. (1998). Omega-3 fatty acid concentrates: nutritional aspects and production technologies. Trends in Food Science & Technology, 9, 230–240.

Horrocks LA and Yeo YK. (1999). HEALTH BENEFITS OF DOCOSAHEXAENOIC ACID (DHA). Pharmacological Research, 40, 211–225.

Simopoulos AP. (1999). Essential fatty acids in health and chronic disease. The American Journal of Clinical Nutrition, 70, 560–569.

Erdman J, Oria M and Pillsbury L (Institute of Medicine (US) Committee on Nutrition). (2011). Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA). In: Erdman J, Oria M and Pillsbury L (Eds), Nutrition and Traumatic Brain Injury: Improving Acute and Subacute Health Outcomes in Military Personnel. The National Academic Press, Washington, DC, 188-204.

Crawford MA. (2000). Placental delivery of arachidonic and docosahexaenoic acids: implications for the lipid nutrition of preterm infants. The American Journal of Clinical Nutrition, 71, 275-284.

Uauy R and Dangour AD. (2008). Nutrition in Brain Development and Aging: Role of Essential Fatty Acids. Nutrition Reviews, 64, 24–33.

Belarbi EH, Molina E and Chisti Y. (2000). A process for high yield and scaleable recovery of high purity eicosapentaenoic acid esters from microalgae and fish oil. Enzyme and Microbial Technology, 26, 516–529.

Ratledge C and Wynn JP. (2002). The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms. Advances in Applied Microbiology, 51, 1–51.

Sahena F, Zaidul ISM, Jinap S, Saari N, Jahurul HA, Abbas KA and Norulaini NA. (2009). PUFAs in Fish: Extraction, Fractionation, Importance in Health. Comprehensive Reviews in Food Science and Food Safety, 8, 59–74.

Huerlimann R, Nys R de and Heimann K. (2010) Growth, lipid content, productivity and fatty acid composition of tropical microalgae for scale-up production. Biotechnology and Bioengineering, 107, 245–257.

Sánchez Mirón A, Cerón Garcı́a MC, Garcı́a Camacho F, Molina Grima E and Chisti Y. (2002). Growth and biochemical characterization of microalgal biomass produced in bubble column and airlift photobioreactors: studies in fed-batch culture. Enzyme and Microbial Technology, 31, 1015–1023.

Molina Grima E, Belarbi EH, Acién Fernández FG, Robles Medina A and Chisti Y. (2003). Recovery of microalgal biomass and metabolites: process options and economics. Biotechnology Advances, 20, 491–515.

Liang Y, Sarkany N and Cui Y. (2009). Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions. Biotechnology Letters, 31, 1043–1049.

Folch J, Lees M and Stanley GHS. (1957). A Simple Method for the Isolation and Purification of Total Lipides from Animal Tissues. Journal of Biological Chemistry, 226, 497–509.

Kołakowska A, Domiszewski Z, Kozłowski D and Gajowniczek M. (2006). Effects of rainbow trout freshness on n-3 polyunsaturated fatty acids in fish offal. European Journal of Lipid Science and Technology, 108, 723–729.

Jiménez Callejón MJ, Robles Medina A, González Moreno PA, Esteban Cerdán L, Orta Guillén S and Molina Grima E. (2020). Simultaneous extraction and fractionation of lipids from the microalga Nannochloropsis sp. for the production of EPA-rich polar lipid concentrates. Journal of Applied Phycology, 32, 1117–1128.

Petkov G and Garcia G. (2007). Which are fatty acids of the green alga Chlorella? Biochemical Systematics and Ecology, 35, 281–285.

Patil V, Källqvist T, Olsen E, Vogt G and Gislerød HR. (2007). Fatty acid composition of 12 microalgae for possible use in aquaculture feed. Aquaculture International, 15, 1–9.

Patel VK, Sundaram S, Patel AK and Kalra A. (2018). Characterization of Seven Species of Cyanobacteria for High-Quality Biomass Production. Arabian Journal for Science and Engineering, 43, 109–121.

Verma NM, Mehrotra S, Shukla A and Mishra BN. (2010). Prospective of biodiesel production utilizing microalgae as the cell factories: A comprehensive discussion. African Journal of Biotechnology, 9, 1402–1411.

Řezanka T, Dor I, Prell A and Dembitsky VM. (2003). Fatty acid composition of six freshwater wild cyanobacterial species. Folia Microbiologica, 48, 71–75.

de Swaaf M, Pronk J and Sijtsma L. (2003). Fed-batch cultivation of the docosahexaenoic-acid-producing marine alga Crypthecodinium cohnii on ethanol. Applied Microbiology and Biotechnology, 61, 40–43.

Jiang Y and Chen F. (1999). Effects of salinity on cell growth and docosahexaenoic acid content of the heterotrophic marine microalga Crypthecodinium cohnii. Journal of Industrial Microbiology and Biotechnology, 23, 508–513.

Poisson L and Ergan F. (2001). Docosahexaenoic acid ethyl esters from Isochrysis galbana. Journal of Biotechnology, 91, 75–81.

Liu LN, Chen XL, Zhang YZ and Zhou BC. (2005). Characterization, structure and function of linker polypeptides in phycobilisomes of cyanobacteria and red algae: An overview. Biochimica et Biophysica Acta (BBA) – Bioenergetics, 1708, 133–142.

Chen GQ, Jiang Y and Chen F. (2007). Fatty acid and lipid class composition of the eicosapentaenoic acid-producing microalga, Nitzschia laevis. Food Chemistry, 104, 1580–1585.

Sahin D, Tas E and Altindag UH. (2018). Enhancement of docosahexaenoic acid (DHA) production from Schizochytrium sp. S31 using different growth medium conditions. AMB Express, 8, 7.

Ahlgren G, Gustafsson IB and Boberg M. (1992). Fatty Acid Content and Chemical Composition of Freshwater Microalgae. Journal of Phycology, 28, 37–50.

Dawczynski C, Schubert R and Jahreis G. (2007). Amino acids, fatty acids, and dietary fibre in edible seaweed products. Food Chemistry, 103, 891–899.

Calder PC and Yaqoob P. (2009). Omega‐3 polyunsaturated fatty acids and human health outcomes. BioFactors, 35, 266–272.

Lopaschuk GD, Ussher JR, Folmes CDL, Jaswal JS and Stanley WC. (2010). Myocardial Fatty Acid Metabolism in Health and Disease. Physiological Reviews, 90, 207–258.

Brostow DP, Odegaard AO, Koh WP, Duval S, Gross MD, Yuan JM and Pereira MA. (2011). Omega-3 fatty acids and incident type 2 diabetes: the Singapore Chinese Health Study. The American Journal of Clinical Nutrition, 94, 520–526.

Tiwari A and Kiran T. (2018). Biofuels from Microalgae. In: Nageswara-Rao M and Soneji JR (Eds), Advances in Biofuels and Bioenergy. IntechOpen, 239-249.