Discriminate and indiscriminate use of ciprofloxacin antibiotic and detection of ciprofloxacin residues in edible poultry tissues

Md Anaruzzaman; Md Shafiqul Islam; Md Rakibul Hasan; DM Nazmul Hossain; Kazi Rafiqul Islam

doi:10.30574/gscarr.2021.6.3.0054

Authors

Md Anaruzzaman Department of Pharmacology, Faculty of Veterinary Science. Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Md Shafiqul Islam Department of Pharmacology, Faculty of Veterinary Science. Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Md Rakibul Hasan Department of Pharmacology, Faculty of Veterinary Science. Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
DM Nazmul Hossain Department of Pharmacology, Faculty of Veterinary Science. Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Kazi Rafiqul Islam Department of Pharmacology, Faculty of Veterinary Science. Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

DOI:

https://doi.org/10.30574/gscarr.2021.6.3.0054

Keywords:

Antibiotic residues, Broiler, Thin layer chromatography, Ciprofloxacin

Abstract

Abuse of antibiotics is more common in developing countries. This study was undertaken to investigate the presence of ciprofloxacin residue in broiler tissues and effects of antibiotics misuse on hematological parameters in broilers. Day old chicks (DOC) were collected and reared up to 31 days. The treatment was started from 16th day until sacrifice. On day 14th, the chicks were randomly divided into three groups namely control group (group A), discriminate antibiotic group (group B), and indiscriminate antibiotic group (group C). The control group was left untreated whereas, the discriminate group was treated with antibiotic, ciprofloxacin, followed by withdrawal period of one week. On the other hand antibiotic treatment was continued in the indiscriminate antibiotic group until the day of sacrifice, without maintaining withdrawal period. The mean body weight gain in treatment period (14 days) was the highest in discriminate group (1312± 26.1gm) followed by indiscriminate group (1089± 222.8gm) and control group (823.3±90.2gm). The Thin Layer Chromatography revealed that all the samples were positive in indiscriminate group with an exception of fat tissue (66.7%). The 50% liver and 33.3% kidney was ciprofloxacin positive in discriminate group and all others were negative. There was no positive sample in control group. . The hematological parameters such as Hb, PCV and TEC values of treatment groups showed significantly (p<0.05) lower, while PCV did not show any significant difference compared to the control group. Hence this experiment identifies the potential effects of ciprofloxacin misuse in broilers and their subsequent impacts on hemolytic system.

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