Thin layer chromatographic detection of colistin sulfate antibiotic residues in poultry tissues

Md. Nazmul Hasan; Md. Shafiqul Islam; Md. Rakibul Hasan; Kazi Rafiqul Islam

doi:10.30574/gscarr.2021.7.1.0063

Authors

Md. Nazmul Hasan Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Md. Shafiqul Islam Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Md. Rakibul Hasan Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Kazi Rafiqul Islam Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

DOI:

https://doi.org/10.30574/gscarr.2021.7.1.0063

Keywords:

Colistin sulfate, Antibiotic residue, TLC, Broiler

Abstract

Residues of antibiotic drugs in food and food products have been received much attention in recent years because of growing food safety concerns for public health. There are serious effects of antibiotic residues in meat for human consumption (e.g., increasing antimicrobial resistance, carcinogenicity, mutagenicity, and hypersensitivity). In this study, we used broiler chicks as a laboratory animal to detect the colistin sulfate antibiotic residue for human health concerns. The day-old broiler chicks were collected and reared for up to 31 days. The treatment was started from the day 16th until sacrifice. The chicks were randomly divided into three groups namely control group (Group A), discriminate antibiotic group (Group B) and indiscriminate antibiotic group (Group C) on the 14th day. The discriminate group was treated with an antibiotic, colistin sulfate maintaining the withdrawal period of one week. In case of indiscriminate group the withdrawal period was not maintained and antibiotic treatment was continued till the day of sacrifice. The body weights of the birds were recorded daily. The mean body weight was highest in indiscriminate group (1261.15±16.37gm) followed by discriminate group (1156.15±18.23gm) and the lowest was in control group (1008.49±18.11gm). The differences among mean weight gain were statistically significant (P<0.05) in antibiotic treated group compared with control group. The Thin Layer Chromatography revealed that all the samples were positive in indiscriminate group and in case of Liver, Kidney and Spleen, it was 100%. In case of discriminate antibiotic group all the samples were positive except thigh and breast muscle and the percentage was highest in Liver sample (66.67%). There was no positive sample in control group. The results were statistically significant (P<0.05). From the above findings, this research could be considered a need based research in Bangladesh to ascertain the influential effect of antibiotic abuse in poultry industry.

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