Analysis of Phenol, Reducing Sugar, and Chlorophyll of Dendrobium sp. plantlet after induction of fusaric acid In Vitro

Endang Nurcahyani 1, *, Elsi Diana 2, Yuliana Permata Sari 2, Sumardi 2, Hardoko Insan Qudus 3 and Mahfut 4

1 Applied Biology Study Program, Faculty of Mathematics and Natural of Sciences, University of Lampung, Bandar Lampung, Lampung, Indonesia.
2 Biology Masters Study Program, Faculty of Mathematics and Natural of Sciences, University of Lampung, Bandar Lampung, Lampung, Indonesia.
3 Chemistry Study Program, Faculty of Mathematics and Natural of Sciences, University of Lampung, Bandar Lampung, Lampung, Indonesia.
4 Biology Study Program, Faculty of Mathematics and Natural of Sciences, University of Lampung, Bandar Lampung, Lampung, Indonesia.
 
Research Article
GSC Biological and Pharmaceutical Sciences, 2022, 19(03), 133–138.
Article DOI: 10.30574/gscbps.2022.19.3.0207
Publication history: 
Received on 25 April 2022; revised on 04 June 2022; accepted on 06 June 2022
 
Abstract: 
Orchids are ornamental plants with varied and beautiful flower colors and are much favored by the public. Orchid plants have a higher economic value when compared to other ornamental plants, both as cut flowers and potted flowers. In the process of growth, orchid plants are disturbed, namely Fusarium wilt disease caused by Genus Fusarium which can produce a toxin compound, namely fusaric acid which is widely used in in vitro selection of plants. Plant resistance can be formed through the induction of fusaric acid compounds. Induced resistance is an alternative way to obtain disease-resistant plants because plants are able to create natural resistance mechanisms. The purpose of this study was to determine the concentration of tolerant fusaric acid to select Dendrobium orchid plantlets with optimal growth and specific expression characters of Dendrobium orchid plantlets based on phenol content, reducing sugar content, and total chlorophyll content, chlorophyll a and chlorophyll b. The results of this study indicated that there was an increase in total phenol content, reducing sugar content, and total chlorophyll content, chlorophyll a and chlorophyll b along with the addition of fusaric acid concentration. 
 
Keywords: 
Chlorophyll; Dendrobium sp.; Fusaric Acid; In vitro; Phenol; Reducing Sugar
 
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