Antibacterial properties and phytochemical screening of Tamarix nilotica leaves from Sudan
1 Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Sudan.
2 Department of Biotechnology, Faculty of Science and Technology, Omdurman Islamic University, Sudan.
3 Department of Microbiology, Faculty of Medical Laboratories, Omdurman Islamic University, Sudan.
Research Article
GSC Biological and Pharmaceutical Sciences, 2018, 03(02), 006–010.
Article DOI: 10.30574/gscbps.2018.3.2.0028
Publication history:
Received on 07 April 2018; revised on 21 April 2018; accepted on 04 May 2018
Abstract:
The antibacterial activity of crude extracts of leaves coated with wax and other de-waxed leaves of Tamarix nilotica, against nine human pathogenic bacteria were evaluated using the agar plate well-diffusion method with plant extract concentration of 100 mg/ml. The minimum inhibitory concentration (MIC) was determined. The antibacterial activity was found in the least MIC, as 7.5 mg/ml, belonged to Staphylococcus aureus and Pseudomonas aeruginosa. Most susceptible gram-negative bacteria were P. aeruginosa and least susceptible gram-negative bacteria were E. coli and S. typha. In gram-positive bacteria, most and least susceptible were S. aureus and B. cereus, respectively. These findings should stimulate the research for novel, natural product such as many anti-bacterial agents. The percentage yield of epicuticle wax from Tamarix nilotica leaves, extracted with n-hexane was 0.7%. The results of the preliminary phytochemical analyses of the ethanol extract of the leaves coated with wax and de-waxed leaves of Tamarix nilotica were performed. Steroids, phenolic compounds, saponins, tannins, flavonoids and sugars are present in all of the two samples analyzed except sugars in de-waxed leaves.
Keywords:
Antibacterial; Phytochemical; Tamarix nilotica; Wax and de-waxed leaves
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Copyright © 2018 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0.
