Antimicrobial activity of the biosynthesized silver nanoparticles of Gossypium hirsutum leaves extract
1 Department of Pharmaceutics, H.S.K.College of Pharmacy Bagalkot-587101, Karnataka, India.
2 Department of Pharmacognosy, H.S.K.College of Pharmacy Bagalkot-587101, Karnataka, India.
Research Article
GSC Biological and Pharmaceutical Sciences, 2021, 15(03), 189–198.
Article DOI: 10.30574/gscbps.2021.15.3.0156
Publication history:
Received on 03 May 2021; revised on 04 June 2021; accepted on 07 June 2021
Abstract:
Silver nanoparticles were successfully synthesized using silver nitrate and Gossypium hirsutum leaves extracts by varying the different concentration of aqueous & ethanolic extract. The Gossypium hirsutum leaves extract was containing phytoconstituents like carbohydrates, proteins, glycosides, flavonoids, alkaloids, tannins and phenolic compounds. Formation of Silver Nano Particles was primarily confirmed by colour change of yellow to brownish color. Silver nanoparticle with phytochemicals was confirmed by UV-Visible spectra by observing peak absorption of aqueous and ethanolic SNP at 430.0 nm and 416.0 nm respectively. Dynamic light scattering of the prepared formulations revealed all the formulation were within nano range, The particle size of SNP were in between 282.1nm to 205.7nm& zeta potential -38.66mV to -4.80mv for aqueous extract & particle size of SNP for ethanolic extract were in between 201.7nm to 156.1nm & zeta potential -29.95mV to -25.36mv.SEM showed the bio synthesized SNP were found to be spherical with rough surface & agglomerated. Antimicrobial activity of biosynthesized SNP was evaluated by means of inhibition zone analysis through well diffusion method, where SNP biosynthesized from aqueous and ethanolic extracts of G.hirsutum showed good antimicrobial activity against studied microorganisms.
Keywords:
Gossypium hirsutum; Silver nanoparticles; Antimicrobial activity
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