Cardioprotective effect of the aqueous extract of seeds of Datura metel (Solanaceae) on acute cardiotoxicity induced with doxorubicin in Wistar rats
1. Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon.
2. Institute of Agricultural Research for Development, P.O. Box 167, Meyomessala, Cameroon.
3. Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, P.O. Box 1364, Yaoundé, Cameroon.
Research Article
GSC Biological and Pharmaceutical Sciences, 2020, 13(03), 008-018.
Article DOI: 10.30574/gscbps.2020.13.3.0350
Publication history:
Received on 28 October 2020; revised on 28 November 2020; accepted on 01 December 2020
Abstract:
The current study evaluated the cardioprotective effect of the aqueous extract of seeds of Datura metel (AESDM) on the acute cardiotoxicity induced with doxorubicin in the Wistar rats. Indeed, 30 rats have been randomized then, divided into 6 groups of 5 animals each: 3 control groups (normal, negative and positive) and 3 test groups (AESDM 100, 200 and 300 mg/kg). These animals received orally, six consecutive days, 1 mL/200 g distilled water (normal and negative control), 100 mg/kg Vitamin-E (positive control), AESDM at 100, 200 and 300 mg/kg (test groups). On the seventh day of treatment, animals in the different groups excepted the normal control received a single intraperitoneal injection of 15 mg/kg doxorubicin followed respective treatments up to the tenth day of the trial period. Serum parameters (ASAT/ALAT), biochemical markers of oxidative stress (MDA, SOD, CAT, GSH) and lipid parameters (total cholesterol, triglycerides, HDL and LDL) were evaluated. Histological sections of animal hearts were prepared. The results of this study showed that treatment with AESDM resulted in a significant decrease (P ˂ 0.001) in MDA, triglycerides and LDL-cholesterol levels, ASAT/ALAT activity and a significant increase (P ˂ 0.001) in HDL-cholesterol concentration and SOD, CAT and GSH activities, compared to the negative control. Histological observation revealed that AESDM protected the heart from doxorubicin-induced damage. In conclusion, AESDM would have a cardioprotective effect that could be attributed to its antioxidant potential.
Keywords:
Datura metel; Cardioprotection; Cardiotoxicity; Doxorubicin; Antioxydant
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