Comparative analysis of total flavonoid, total phenolic content, and antioxidant activity of Theobroma cacao Pod Husk extract and its derived fractions
Department of Medical Laboratory Technology, Poltekkes Kemenkes Tanjungkarang, Indonesia & Center of Excellence Safe Community for Public Health in Agroindustry Area, Poltekkes Kemenkes Tanjungkarang, Indonesia.
Research Article
GSC Biological and Pharmaceutical Sciences, 2023, 25(03), 159–166.
Article DOI: 10.30574/gscbps.2023.25.3.0525
Publication history:
Received on 06 November 2023; revised on 18 December 2023; accepted on 21 December 2023
Abstract:
Secondary metabolites from plants are known to be promising sources of raw materials for drugs in terms of safety. This research focuses on cacao pod husk as a candidate for discovering natural antioxidant compounds. The stages in this study include the extraction of cacao pod husk using methanol as a solvent, followed by liquid-liquid fractionation to produce ethyl acetate and acetone fractions. Subsequently, total flavonoid and phenolic content, DPPH antioxidant activity tests, and GCMS metabolite analysis were conducted. The research results indicate that the ethyl acetate fraction has the highest total flavonoid and phenolic content compared to methanol extract and acetone fraction. The methanol extract has the highest antioxidant activity with an IC50 of 0.0736 mg/mL. The identified metabolites belong to the groups of polyphenols, flavonoids, steroids, and their derivatives, as well as some volatile compounds such as hexadecanoic acid methyl ester. Compounds with potential antioxidant properties include Eugenol, Myristicine, Piperine, and Theobromine.
Keywords:
Cacao pod husk; DPPH; Antioxidant; Cacao
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Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
