Comparative study of aqueous and alcoholic extract of Azadirachta indica A. Juss. (Meliaceae) leaves on two life stages of Haemonchus contortu
1 Normale High Scool /01 BP 1757 Ouagadougou 01/ Institute of Science and technology, Ouagadougou, Burkina Faso.
2 University Joseph KI-ZERBO /03 BP 7021 Ouagadougou 03/Departement of Animale Biology and Animal Physiology/ Physiology Animal Laboratory, Burkina Faso.
3 Université Saint Thomas d'Aquin/ 06 BP: 10212 Ouagadougou 06, Ouagadougou, Burkina Faso.
4 National Center for Scientific and Technological Research (CNRST)/ Department of Animale Production/ 04 BP 8645 Ouagadougou 04, Burkina Faso.
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 26(02), 169–178.
Article DOI: 10.30574/gscbps.2024.26.2.0062
Publication history:
Received on 02 January 2024; revised on 13 February 2024; accepted on 15 February 2024
Abstract:
In Burkina Faso, the production of small ruminants is confronted with many constraints, including gastrointestinal parasitosis. These parasitic diseases causes many mortality in small ruminant breeding therefore considerable economic losses. Thus, the present study was carried out with the aim of evaluating the in vitro anthelmintic effectiveness of Azadiracta indica (A. indica) leaves on two life stages of Haemonchus contortus (H. contortus): eggs and adult worms. To do this, an aqueous and ethanolic extract of A. indica leaves was carried out. From these extracts, five (5) increasing concentrations were performed: 1.25; 2.5; 5 ; 10 and 20 mg/mL and three (3) controls (distilled water, DMSO 2% and albendazol). These concentrations were brought into contact with i) eggs for Eggs Hatch inhibition Assay st (EHA) and ii) Adult Mortality Test (AMT). Three replicates were performed for each experimental preparations. In vitro observations showed that ethanolic extract of A. indica caused high mortalities (p < 0.0001) ranging from 44.44 to 100% at 2 hours and 6 hours of exposure respectively at 20 mg/mL. In the same time interval, albendazol showed 100% of mortality in H. contortus. For EHA, the inhibition rate varied from 18% to 85% for the aqueous extract and from 54% to 93% for ethanolic extract. We can therefore say that A. indica extracts have nematicidal and ovicidal effect.
Keywords:
Azadirachta indica; Haemonchus contortus; Small ruminants; Gastrointestinal; Anthelmintic
Full text article in PDF:
Copyright information:
Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
