Contribution of garlic for improving the cytoprotective effect of mesna against cyclophosphamide toxicity in rats
1 Department of Pathology, Faculty of Veterinary medicine, University of Sadat City, Egypt.
2 Department of Forensic Medicine and Toxicology, Faculty of Veterinary medicine, University of Sadat City, Egypt.
3 Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary medicine, University of Sadat City, Egypt.
4 Department of Pharmacology, Faculty of Veterinary medicine, University of Sadat City, Egypt.
Research Article
GSC Biological and Pharmaceutical Sciences, 2019, 07(03), 008–020.
Article DOI: 10.30574/gscbps.2019.7.3.0089
Publication history:
Received on 16 May 2019; revised on 27 May 2019; accepted on 03 June 2019
Abstract:
Cyclophosphamide (CYP), an oxazophosphorine- alkylating agent, is a widely used drug for treatment of neoplastic and severe autoimmune diseases. Adverse effects of CYP restrict its therapeutic benefits. Mesna (MS), a thiol compound, is used as a protective agent against hemorrhagic cystitis induced by CYP. This study aimed to investigate the potential role of raw garlic homogenate (RGH) for improving the protective effect of mesna against CYP toxicity in rats. Thirty male albino rats were divided into five equal groups. Control group, CYP group (a single i.p. dose, 200 mg/kg b.wt.), CYP+MS group (a total dose of 120 mg/kg b.wt. in three equal doses, i.p.), CYP+RGH group (500 mg/kg b.wt., orally, once daily 5 days before and after CYP injection) and CYP+MS+RGH group. CYP induced hematological changes, including significant reduction of RBCs, Hb, PCV with thrombocytopenia and leukocytopenia. Also, significant increase in serum MDA content concomitantly with significant reduction in TAC was recorded. This was associated with histopathological alterations in the examined tissues. However, mesna and/or garlic improved the recorded hematological, biochemical and histopathological alterations induced by CYP with marked improvement using their combination. In conclusion, garlic supplementation in combination with mesna could be of a great value to introduce therapeutic strategies for patients undertaking cyclophosphamide therapy.
Keywords:
Cyclophosphamide; Garlic; Mesna; Hematology; Histopathology
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Copyright © 2019 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
