Determination of polyphenolic components by high performance liquid chromatography (HPLC) and evaluation of the antioxidant activity of leaves and fruits of Crataegus mongyna Jacq
1 Laboratory of Pharmacognosy, Department of Pharmacy, Faculty of Medicine, Badji Mokhtar-Annaba University, Algeria.
2 Laboratory of Development and Control of Pharmaceutical Preparations, Department of Pharmacy, Faculty of Medicine, Badji Mokhtar-Annaba University, Algeria.
Research Article
GSC Biological and Pharmaceutical Sciences, 2020, 13(01), 251-256.
Article DOI: 10.30574/gscbps.2020.13.1.0344
Publication history:
Received on 16 October 2020; revised on 27 October 2020; accepted on 28 October 2020
Abstract:
Crataegus mongyna Jacq is an endemic species of eastern Algeria, used in traditional medicine for its many therapeutic virtues. Its leaves and fruits are rich in polyphenols. In the present study we wanted to research its polyphenolic components in which it is rich. The method we used is high performance liquid chromatography (HPLC) and evaluation of the antioxidant activity in the methanolic extracts of its leaves and fruits. For this evaluation we followed the free radical reduction method DPPH (2, 2-diphenyl-1-picryl hydrazyl) by an antioxidant. The results show, the presence of polyphenolic components such as rutin, quercetin and isoquercetin and significant antioxidant activity. Crataegus monogyna Jacq is could be placed as an alternative treatment for certain pathologies because it is of value for public health.
Keywords:
Crataegus mongyna Jacq; Leaves; Fruits; Methanolic extracts; Polyphenolic components; Antioxidant activity
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