The effect of Centella asiatica ethanolic extract on expression of glucose transporter 1 and osteocalcin on stunting larvae of Zebrafish (Danio rerio)

Yuningsih 1, 2, Dyah Woro Kartiko Kusumo Wardani 1, 3, Mulyohadi Ali 4, Husnul Khotimah 4, *, Nurdiana 4, Wibi Riawan 5, Dianita Primihastuti 1, 6, Fitra Arsy Nur Cory’ah 1, 7, Linda Ika Puspita Ariati 1, 8, Vanda Primaditya 1, 9 and Zakiah 1, 10

1 Midwifery Programe, Faculty of Medicine, Universitas Brawijaya, Indonesia.
2 Midwifery Programe, Universitas dr.Soebandi Jember, East Java, Indonesia.
3 Midwifery Programe, STIK Sint Carolus, Jakarta, Indonesia.
4 Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, Jawa Timur, Indonesia.
5 Department of Biochemistry-Biomolecular Faculty of Medicine, Universitas Brawijaya, Malang, Jawa Timur, Indonesia.
6 Midwifery Programe, STIKES William Booth Surabaya, Surabaya, Jawa Timur, Indonesia.
7 Midwifery Programe, Politeknik Kesehatan KEMENKES Mataram, NTB, Indonesia.
8 Midwifery Programe, Kebidanan Academy Jember, Jember, East Java, Indonesia.
9 Midwifery Program, Faculty of Medicine, Universitas Brawijaya, Indonesia
10 University of Darul Ulum Jombang High Islamic Boarding School, Jombang, East Java, Indonesia.
 
Research Article
GSC Biological and Pharmaceutical Sciences, 2022, 19(03), 178–189.
Article DOI: 10.30574/gscbps.2022.19.3.0230
Publication history: 
Received on 10 May 2022; revised on 13 June 2022; accepted on 15 June 2022
 
Abstract: 
Stunting is known as length for age below minus two standard deviations (<-2 SD) of the WHO Child Growth Standard nutritional status. Several causes are identified, including exposure to pesticides (rotenone), which works by inhibiting mitochondrial complex I as an ATP-producing site and generate oxidative stress that affects glucose delivery and bone elongation involving GLUT 1 and osteocalcin. Centella asiatica is a high antioxidant herbal plant with anti-inflammatory properties. This study aimed to investigate the effect of Centella asiatica ethanolic extract on the rotenone-induced stunting zebrafish (Danio rerio) larvae model through GLUT 1 and osteocalcin expressions. This study found that 12.5 ppb of rotenone can induce about 6 dpf (day post-fertilization) zebrafish larvae into stunting. The effect of Centella asiatica ethanolic extract on increasing body length in stunting zebrafish larvae works by increasing the expression of GLUT 1 and osteocalcin expressions. The correlation test showed a positive and strong relationship between this ethanolic extract level with GLUT 1 and Osteocalcin expression. Thus, the Centella asiatica ethanolic extract effectively promotes the body length by elevating the expression of GLUT 1 and osteocalcin in stunting zebrafish larvae.
 
Keywords: 
Centella asiatica; Rotenone; Glucose Transporter 1; Osteocalcin; Stunting; Zebrafish
 
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Copyright information: 
Copyright © 2022 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0

 

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