Effect of Dihydroartemisinin on contractility to Drugs in isolated smooth muscle Preparations in guinea pig in vitro models
1 Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Uyo Akwa Ibom State, Nigeria.
2 Department of Pharmacology and Therapeutics College of Health Sciences, Nnamdi Azikiwe University, Nnewi, Anambra State, Nigeria.
Research Article
GSC Biological and Pharmaceutical Sciences, 2023, 23(03), 083–091.
Article DOI: 10.30574/gscbps.2023.23.3.0191
Publication history:
Received on 05 April 2023; revised on 04 June 2023; accepted on 07 June 2023
Abstract:
This study was aimed at evaluating the effect of Dihydroartemisinin (DHA)on contractility to drugs in ileum isolated smooth muscle preparations in guinea pigs using in Vitro experimental procedures / standard protocols in an organ bath set up. dihydroartemisinin (1.0 x 10 - 8 - 1.0 x 10 – 5mg/mL) when applied alone and separately excited marked variable effects on guinea pig ileum . In some preparation it showed no response, while in others it produced slight phasic contraction when external calcium (Ca2+) ion was introduced. The slight phasic contractile activity was abolished by verapamil (5x10-3mg/mL). Dihydroartemisinin (1.0 x 10 - 6 - 1.0 x 10 – 5mg/mL) caused marked significant induced contraction – dependent inhibition of acetylcholine, potassium chloride and histamine in depolarizing tyrode solution (p < 0.05).The Inhibitory response maxima of dihydroartemisinin on acetylcholine induced contraction is 17.0±0.1 mm(65.56% inhibition) ; and is moderate when compared to the inhibitory effect of atropine (10-5 M). The result shows that dihydroartemisinin seems to act via non-specific receptor mechanism, with appreciable calcium channel blocking activity and it is safe at therapeutic doses.
Keywords:
Dihydroartemisinin; Contractility; Smooth muscle; Plasmodiumberghei bergiei,in vitro; toxicity
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