Effects of Borassus flabellifer L. fruit seed coat on HSC-3 cell line
Oral Pathology Department, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia.
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 26(02), 033–037.
Article DOI: 10.30574/gscbps.2024.26.2.0051
Publication history:
Received on 27 December 2023; revised on 03 February 2024; accepted on 05 February 2024
Abstract:
Background: The seed coat of Borassus flabellifer L. fruit contains antioxidants that can neutralize the toxic effects of free radicals and can fight DNA damage due to exposure to oxidative stress. The extract of B. flabellifer is proven to have cytotoxic effects and can inhibit the growth of several types of cancer cells such as HeLa cell lines.
Objective: To determine the cytotoxicity effect of extract of B. flabellifer L. fruit seed coat against HSC-3 cell line. Methods: Laboratory experimental research was conducted using HSC-3 cell lines treated with B. flabellifer L. fruit seed coat extract at concentrations of 93.75 µg/mL, 187.5 µg/mL, 375 µg/mL, 750 µg/mL, and 1,500 µg/mL, negative control DMEM-FBS 20%, and positive control anti cancer drugs Doxorubicin 3 µM. HSC-3 cell line cytotoxicity assay was performed using CCK-8 reagent. Micro plate reader at 450 nm wavelength was used to determine the viability of HSC-3 cell lines by measuring the optical density of formazan.
Results: B. flabellifer fruit L. seed coat extract was able to reduce viability and was cytotoxic to HSC-3 cell lines at concentrations of 93.75 µg/mL, 187.5 µg/mL, 375 µg/mL, 750 µg/mL, and 1,500 µg/mL, with an IC50 value of 141.9 µg/mL.
Conclusion: B. flabellifer L. fruit seed coat extract can reduce the ability of HSC-3 cell line cancer cells to survive by inhibiting cell proliferation due to its cytotoxicity effect.
Keywords:
Borassus flabellifer L; Fruit seed coat; Cancer cell; HSC-3 cell line; Cytotoxicity; CCK-8.
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Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
