Effects of Platostoma palustre ethanolic extracts and commercial herbal tea on the cell viability of colorectal cancer cells
1 Bachelor Degree Program in Pet Healthcare, Yuanpei University of Medical Technology, Xiangshan, Hsinchu 300, Taiwan.
2 Division of Animal Industry, Animal Technology Research Center, Agricultural Technology Research Institute, Xiangshan, Hsinchu 300, Taiwan.
3 Graduate Institute of Metabolism and Obesity Sciences, College of Nutrition, Taipei Medical University, Taipei 110, Taiwan.
4 Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
5 Department of Science and Technology, Council of Agriculture, Executive Yuan, Taipei 100, Taiwan.
6 Department of Nursing, Yuanpei University of Medical Technology, Hsinchu 300, Taiwan.
# Contributed equally to this work.
Research Article
GSC Biological and Pharmaceutical Sciences, 2022, 18(02), 326–330.
Article DOI: 10.30574/gscbps.2022.18.2.0086
Publication history:
Received on 21 January 2022; revised on 24 February 2022; accepted on 26 February 2022
Abstract:
Platostoma palustre jelly is a traditional food. Platostoma palustre has been used as folk medicine and is effective against heat-shock, hypertension and diabetes. Therefore, the aim of this study was to determine the effects of ethanolic extracts and commercial herbal tea of Platostoma palustre in inhibiting colorectal cancer cell viability. The ethanolic extracts of Platostoma palustre by using 90% ethanol for extraction. In this study, 2-fold serial dilution of 100 mg/mL Platostoma palustre extracts were applied. On other hand, the same dilution fold was also performed for 100% commercial herbal tea with Platostoma palustre. Additionally, CT-26 and HT-29 colorectal cancer cell lines were also used in this study. After co-culturing for 24 hours, the cell viability of CT-26 and HT-29 colorectal cancer cell lines were performed by using 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. According to these data, the 1.56-100 mg/mL Platostoma palustre extracts possessed the significant inhibition effects of CT-26 colorectal cancer cell viability. The 3.13-100% commercial herbal tea with Platostoma palustre possessed the significant inhibition effects of CT-26 colorectal cancer cell viability. The 6.25-100 mg/mL Platostoma palustre extracts possessed the significant inhibition effects of HT-29 colorectal cancer cell viability. The 25-100% commercial herbal tea with Platostoma palustre possessed the significant inhibition effects of HT-29 colorectal cancer cell viability. However, the 0.39-3.13 mg/mL Platostoma palustre extracts possessed the significant promoting effects of HT-29 colorectal cancer cell viability. The 0.39-12.5% commercial herbal tea with Platostoma palustre also possessed the significant promoting effects of HT-29 colorectal cancer cell viability. Comparison of CT-26 and HT-29 cell lines was on the cell viability after Platostoma palustre ethanolic extracts and commercial herbal tea treatments, CT-26 cell line was better sensitive than HT-29 cell line on the inhibition of cell viability after treatment of Platostoma palustre ethanolic extracts and the commercial herbal tea. Taken these results together, Platostoma palustre ethanolic extracts and commercial herbal tea may have a potential for inhibiting the growth of colorectal cancer cells.
Keywords:
Cell viability; Colorectal cancer; Commercial herbal tea; Ethanolic extraction; In vitro; Platostoma palustre extracts
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