ELISA-assay for Pig Taenia solium Cysticercosis by using T18 recombinant antigen
1 Institut Pasteur de Madagascar, Antananarivo, Madagascar.
2 Ecole Doctorale Sciences de la Vie et de l’Environnement; Faculté des Sciences; Université d’Antananarivo; Madagascar.
3 Institut Pasteur de Côte d’Ivoire, Unité d’Immunologie.
4 Faculté des Sciences, Université d’Antananarivo; Madagascar.
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 29(03), 174-182.
Article DOI: 10.30574/gscbps.2024.29.3.0473
Publication history:
Received on 02 November 2024; revised on 13 December 2024; accepted on 16 December 2024
Abstract:
Cysticercosis is caused by infection with the larval stage of tapeworm parasite Taenia solium. This zoonose is a major public health issue with high prevalence in Madagascar, around 21% and 7% to 20% respectively in swine and human. ELISA and Enzyme-linked Immuno-electro Transfer Blot (EITB) remained the main laboratory serological diagnostic tools available in the island. Four native glycoproteins are recognized by antibodies in a majority of sera from patients with cysticercosis and a similar pattern seems to be present in infected pigs. For a better management of this disease in Madagascar, the aim of this study is to set-up and validate the one majority glycoprotein antigen T18 (18KDa) for diagnosis test of pig cysticercosis, produced as recombinant protein.
Using a cDNA library prepared from T. solium cysticerci obtained from different regions of Madagascar, we first analyzed the polymorphism of the T18 gene. Then, gene was cloned into the pMAL-c2X plasmid for expression as an N-terminal maltose-binding protein (MBP) fusion protein and C-terminal hexa-His tagged protein. Product protein was then validated by ELISA test of pig sera.
Preliminary results show a strong conservation of T18 gene. And indeed larger amounts of soluble recombinant protein were obtained from these antigen. T18 recombinant-ELISA present 70% of sensitivity and 100% of specificity.
Keywords:
Cysticercosis; ELISA-assay; Pig; Recombinant protein; T18
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