Evaluation of antibacterial property and phytochemical composition of the crude ethanolic extract from Kalingag (Cinnamomum mercadoi) barks

Maxime Benedict Paguirigan Tirol 1, Francis Augustine Autor Bajao 1, Pamela Guillena Biwang 1, Joenly Rose Avendaño Cardenas 1, Al-Farok Calandada Casidar 1, John Miguel Petallar Chiong 1, Sweet Elise Pelaez Labnao 1, Jovelyn Laurente Lacubtan 1, Hanagen Liclican Salvador 1, Madeleine Cabeltes Zamayla 1, Jevie Lyn Peralta-Tan Nery 2, Shosi Mari Salubre Larido 3, Sandra Enriquez Laguimun 6, 7, Rowel Paningbatan Catchillar 4, Justin Dave Magracia Manantan 5, 6, * and Jan Karlo Tiongson Ecalne 1, 3, 6, 7

1 Allied Health Program, Lourdes College, Capistrano Street, Cagayan de Oro, 9000, Philippines.
2 College of Allied Health Science Education, Jose Maria College Foundation Inc., Philippine-Japan Friendship Highway, Davao City, 8000, Philippines.
3 College of Pharmacy, Medina College Ipil, Inc., Don Andres, Ipil, Zamboanga Sibugay, 7001, Philippines.
4 College of Pharmacy, Virgen Milagrosa University Foundation, Martin P. Posadas, Avenue, San Carlos City, Pangasinan, 2420, Philippines.
5 College of Pharmacy, Adamson University, 900 San Marcelino Street, Ermita, Manila, 1000, Philippines.
6 Graduate School, Adamson University, 900 San Marcelino Street, Ermita, Manila, 1000, Philippines.
7 School of Pharmacy, Centro Escolar University – Manila, 1001 San Rafael Street, San Miguel, Manila, 1005, Philippines.
 
Research Article
GSC Biological and Pharmaceutical Sciences, 2024, 28(01), 156–163.
Article DOI: 10.30574/gscbps.2024.28.1.0265
Publication history: 
Received on 08 June 2024; revised on 18 July 2024; accepted on 20 July 2024
 
Abstract: 
Cinnamomum mercadoi, locally known as Kalingag, is a native plant of the Philippines that has limited studies despite its long use in traditional Philippine medicine due to its indigenous origin. This study examined the phytochemical composition and antibacterial activities of a crude ethanolic extract derived from Kalingag barks. The plant bark gathered from Davao Oriental was thoroughly rinsed with distilled water to eliminate any attached dirt, oven-dried for 6 hours, and then pulverized using a Wiley mill. The bark was then subjected to Soxhlet extraction, and the pooled extracts were concentrated for 5 hours at 40°C to produce a green, syrupy substance. The extract was tested qualitatively, followed by FTIR analysis to confirm the results, before being subjected to the agar well diffusion method to evaluate its antibacterial properties against Staphylococcus aureus and Escherichia coli. The phytochemical evaluation and subsequent FTIR analysis revealed that the extract contained various phytochemicals, including glycosides, tannins, phytosterols, and terpenoids. The antibacterial testing revealed concentration-dependent inhibition that could be improved by purifying or isolating specific compounds from the extract. Additional research into other plant parts is suggested to enhance its biomedical and therapeutic applications.
 
Keywords: 
Antibacterial; Bark; Cinnamomum mercadoi; Agar well
 
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Copyright information: 
Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0

 

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