Evaluation of qualitative and quantitative phytochemical constituents of Napoleona imperialis stem bark

 Oluwayemisi  Odeyemi 1, *,  Anthony Uchenna Onochie 2, Sunday Oluwaseun  Odeyemi 3 and Ifeanyi Onyema Oshim

1 Department of Medical Microbiology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Anambra State, Nigeria.
2 Department of Biochemistry, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Uli, Anambra State, Nigeria.
3 Defence Reference Laboratory, Mogadishu Cantonment, Asokoro, Abuja, Federal Capital Territory, Nigeria.
4 Department of Medical Laboratory Science, Faculty of Basic Medicine and Health Sciences, Benson Idahosa University, Benin City, Nigeria.
 
Research Article
GSC Biological and Pharmaceutical Sciences, 2023, 24(01), 202–208.
Article DOI: 10.30574/gscbps.2023.24.1.0246
Publication history: 
Received on 12 May 2023; revised on 10 July 2023; accepted on 13 July 2023
 
Abstract: 
The aim of this study was to determine the qualitative and quantitative phytochemicals of Napoleona imperialis stem bark. Methods: Extractions and Phytochemical analysis of Napoleona imperialis stem bark were done using standard procedures. Result: Aqueous Napoleona imperialis stem bark extract had yield of 76.9g (7.69%) and methanol Napoleona imperialis stem bark extract also yielded 58.5g (5.85%) when 1000g pulverized was used for each. Qualitative phytochemical analysis of this extract exhibited moderate quantities of alkaloid, steroid and terpenoid in aqueous extract while methanol extract had flavonoid, saponin and tannin also in moderate quantities. Scanty quantities of flavonoid, saponin, cardiac glycoside and tannin were seen in aqueous extract so also, scanty quantities of alkaloid, steroid, terpenoid and cardiac glycoside were seen in methanol extract. The quantitative analysis of the phytochemicals was carried out twice and the average taken. Phenol had a concentration of 1.173mg/kg, Steroids had 0.623mg/kg, Terpenoid had 3.031mg/kg, Flavonoid showed 58.240mg/kg, Saponin had 13.190mg/kg, Alkaloid had 13.530mg/kg, Cardiac glycoside with 3.910mg/kg, Phytate showed 2.320mg/kg, Tannin had 52.500mg/kg and Hydrogen cyanide showed13.500mg/kg. This research proves that Napoleona imperialis contains some phytochemicals that are useful for mankind and also the part of this plant extracts exhibit medicinal properties.
 
Keywords: 
Napoleona imperialis; Aqueous extract; Methanol extract; Phytochemical analysis
 
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