Functional and molecular characterization of plant growth promoting bacteria associated with sugarcane cultivated in Tay Ninh Province, Vietnam
1 Department of Natural Science Teacher Training, Sai Gon University, HCM City, Vietnam.
2 Dept. Microbiology Biotechnology, Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam.
Research Article
GSC Biological and Pharmaceutical Sciences, 2020, 11(02), 265-277.
Article DOI: 10.30574/gscbps.2020.11.2.0136
Publication history:
Received on 09 May 2020; revised on 21 May 2020; accepted on 24 May 2020
Abstract:
Bacteria associated with sugarcane were investigated characteristics of plant growth promotion and identified base on molecular characteristics. Nitrogen fixation bacteria were isolated by using N-free Burk’s and LGI media. Pure colonies were then streaked on NBRIP medium to select phosphate solubilizing bacteria. The ability of producing IAA was screened by dropping Salkowski reagent into the bacterial suspension. There were 140 isolates of nitrogen-fixing and phosphate-solubilizing bacteria, and 41 isolates among them showed IAA producing. The abilities of producing IAA, fixing nitrogen, and solubilizing phosphate of these 41 isolates ranged from 1.9 to 12.8 mg L-1 IAA, 0.1 to 2.5 mg L-1 NH4+, and 32.4 - 286.5 mg L-1 P2O5, respectively. Thirteen selected isolates were preliminary tested biological control ability and identified base on 16S rDNA sequencing. There were 4 isolates capable of producing siderophore, 8 isolates inhibit Fusarium sp., 4 isolates inhibit Aspergillus niger, and one isolate capable of resisting both Escherichia coli and Staphylococcus aureus. The identification result showed these isolates belong to genera Stenotrophomonas, Bacillus, Chitinophaga, Burkholderia, Serratia, and Acinetobacter. These outstanding isolates were proposed to test the ability to promote sugarcane growth.
Keywords:
Plant associated bacteria; Plant growth promoting bacteria; Biological control; Nitrogen fixation; Sugarcane.
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Copyright © 2020 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
