Gastroprotective effects of bromelain on indomethacin-induced gastric ulcer in rats
1 Department of Biophysics, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
2 Department of Physiology, Faculty of Medicine, Erzincan Binali Yidirim University, Erzincan, Turkey.
3 Department of Pathology, Faculty of Veterinary, Atatürk University, Erzurum, Turkey.
4 Department of Medical Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey.
Research Article
GSC Biological and Pharmaceutical Sciences, 2023, 23(01), 277–286.
Article DOI: 10.30574/gscbps.2023.23.1.0174
Publication history:
Received on 19 March 2023; revised on 25 April 2023; accepted on 28 April 2023
Abstract:
Gastric ulcer is one of the most common diseases of the gastrointestinal system and some of its complications are the main causes of morbidity and mortality. Bromelain (BRM), a sulfhydryl proteolytic enzyme, has multiple activities in many areas of medicine as an antioxidant and anti-inflammatory agent. In this study, it was aimed to investigate the anti-inflammatory and oxidative stress-mediated therapeutic effect of BRM in a gastric ulcer model induced by indomethacin (IND) which is a non-steroidal anti-inflammatory drug (NSAID) with gastric ulcer-forming effects. Gastric mucosal paraoxonase (PON), arylesterase (ARE), catalase (CAT), myeloperoxidase (MPO) and glutathione peroxidase (GPx) activity and total glutathione (GSH) levels were analyzed. Interleukin-33 (IL-33), the nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1) levels were determined by immunohistochemically. Although IND-induced histopathological changes occurred, BRM pretreatment prevented these effects. As a result of the study, it was determined that BRM had an anti-ulcerative effect at a dose of 50 mg/kg. BRM given at this dose significantly decreased gastric tissue MPO activity, and increased CAT, PON, ARE, GPx activity and GSH level. The increased IL-33 and decreased Nrf-2 and HO-1 levels were obtained in IND group and these changes were reversed by BRM. As a result, it was concluded that BRM may have protective effects in IND-induced gastric model by reducing inflammation and regulating oxidant/antioxidant balance.
Keywords:
Indomethacin; Gastric ulcer; Bromelain; Inflammation; Oxidative stress
Full text article in PDF:
Copyright information:
Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
