Green synthesis of non-cytotoxic silver nanoparticles using Solanum nigrum leaves extract with antibacterial properties
Department of Biotechnology, R.V. College of Engineering, Karnataka, Bangalore-560059, India.
Research Article
GSC Biological and Pharmaceutical Sciences, 2021, 15(03), 262–271.
Article DOI: 10.30574/gscbps.2021.15.3.0170
Publication history:
Received on 11 May 2021; revised on 20 June 2021; accepted on 21 June 2021
Abstract:
Green synthesizes of silver nanoparticles is a promising method in material science and nanotechnology today. Silver nanoparticles (AgNP) were synthesized using a high-efficiency, cost-effective green and simple method at room temperature using Solanum nigrum leaves extract. The biosynthesized AgNP was validated and classified using spectroscopy profiles from ultraviolet-visible spectroscopy, scanning electron microscope, Fourier-transform infrared spectroscopy and x-ray diffraction. FTIR spectra indicated the presence of biological molecules in AgNP synthesis and UV-visible spectra revealed a surface resonance peak of 420 nm corresponding to AgNP formation. The antimicrobial potential of these synthesized nanoparticles is evaluated against the bacteria Escherichia coli (MCC 2079) and Staphylococcus aureus (MCC 2408), both of which are important foodborne pathogens. Ciprofloxacin and Clotrimazole were used as positive controls antibiotic. The nanoparticles were shown to have strong antibacterial efficacy against the strains examined. Their antibacterial function allows them to be included in antimicrobial formulations. By using the MTT assay, the biocompatibility of Solanum nigrum silver nanoparticles were found non-toxic to mouse fibroblast cell lines (L929 is one of the first in continuous culture to be created. The L strain was generated from a male C3H mouse's usual subcutaneous areolar and adipose tissue.) at lower concentrations.
Keywords:
Silver nanoparticle; Solanum nigrum; MTT assay; Mouse fibroblast cell lines
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Copyright © 2021 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
