Hepatotoxicity effects of Methanol extract of Gmelina arborea leaves on liver enzymes marker in Wistar Rats
1 Pharmacology unit, Department of Biochemistry, Micheal Okpara University of Agriculture Umudike Abia State.
2 National Biotechnology Development Agency FCT Abuja.
3 Department Enviromental Management and Toxicology Micheal Okpara University of Agriculture Umudike Abia State.
Research Article
GSC Biological and Pharmaceutical Sciences, 2021, 15(02), 059–066.
Article DOI: 10.30574/gscbps.2021.15.2.0107
Publication history:
Received on 16 March 2021; revised on 04 May 2021; accepted on 07 May 2021
Abstract:
Liver diseases are a major cause of mortality and morbidity in developing countries. This study aims at determining the hepatotoxicity effects of methanolic-extracts of Gmelina arborea leaves at different concentrations on liver marker enzymes in wistar rats. The experiment was laid out in a complete randomized design (CRD). Fresh leaves of Gmelina arborea from the school environments was collected, dried, grinded and extracted using muslin cloth and methanol. The extract was then placed in the oven at about 38oC for dryness. The extract was administered to five groups of wistar rats orally and twice daily for 21 days at doses of 1000mg/kg, 500mg/kg, 250mg/kg, 125mg/kg and group one control group (wasn’t administered this extract). At the end of every week (7 days) the blood samples were collected through ocular puncture, and biochemical analysis of Serum test (Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP) , Total bilirubin (TB) and Conjugated bilirubin (CB) were estimated. There was no mortality observed as a result of administration of this leave extract. Methanol extract of Gmelina arborea did not produce significant differences in the body weight, organ weights and feeding habits between control and treated animals. Pathologically, neither gross abnormalities nor histopathological changes were observed. Methanol extract of Gmelina arborea was found safe in acute and repeated dose toxicity study when tested in wistar rat.
Keywords:
Mortality; Hepatotoxicity; Gmelina arborea; Histopathology; Concentrations.
Full text article in PDF:
Copyright information:
Copyright © 2021 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0
